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dc.contributor.authorBorneloev, Susanne
dc.contributor.authorCzech, Benjamin
dc.contributor.authorHannon, Gregory
dc.date.accessioned2022-05-22T01:06:35Z
dc.date.available2022-05-22T01:06:35Z
dc.date.issued2022-04-19
dc.identifier.issn2041-1723
dc.identifier.other35440552
dc.identifier.otherPMC9018710
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/337370
dc.description.abstractPIWI-interacting RNAs (piRNAs) are small RNAs required to recognize and silence transposable elements. The 5' ends of mature piRNAs are defined through cleavage of long precursor transcripts, primarily by Zucchini (Zuc). Zuc-dependent cleavage typically occurs immediately upstream of a uridine. However, Zuc lacks sequence preference in vitro, pointing towards additional unknown specificity factors. Here, we examine murine piRNAs and reveal a strong and specific enrichment of three sequences (UAA, UAG, UGA)-corresponding to stop codons-at piRNA 5' ends. Stop codon sequences are also enriched immediately after piRNA processing intermediates, reflecting their Zuc-dependent tail-to-head arrangement. Further analyses reveal that a Zuc in vivo cleavage preference at four sequences (UAA, UAG, UGA, UAC) promotes 5' end stop codons. This observation is conserved across mammals and possibly further. Our work provides new insights into Zuc-dependent cleavage and may point to a previously unrecognized connection between piRNA biogenesis and the translational machinery.
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourcenlmid: 101528555
dc.sourceessn: 2041-1723
dc.subjectAnimals
dc.subjectMammals
dc.subjectMice
dc.subjectEndoribonucleases
dc.subjectDrosophila Proteins
dc.subjectRNA, Small Interfering
dc.subjectCodon, Terminator
dc.titleAn evolutionarily conserved stop codon enrichment at the 5' ends of mammalian piRNAs.
dc.typeArticle
dc.date.updated2022-05-22T01:06:34Z
prism.issueIdentifier1
prism.publicationNameNat Commun
prism.volume13
dc.identifier.doi10.17863/CAM.84784
dcterms.dateAccepted2022-03-03
rioxxterms.versionofrecord10.1038/s41467-022-29787-3
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidBorneloev, Susanne [0000-0001-9276-9981]
dc.contributor.orcidCzech, Benjamin [0000-0001-8471-0007]
dc.contributor.orcidHannon, Gregory [0000-0003-4021-3898]
dc.identifier.eissn2041-1723
pubs.funder-project-idWellcome Trust (110161/Z/15/Z)
pubs.funder-project-idRoyal Society (RSRP\R\200001)
pubs.funder-project-idCancer Research UK (C14303/A17197)
pubs.funder-project-idCancer Research UK (C9545/A29580_do not transfer)
cam.issuedOnline2022-04-19


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International