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dc.contributor.authorAlawami, Mohammed F
dc.contributor.authorBošković, Filip
dc.contributor.authorZhu, Jinbo
dc.contributor.authorChen, Kaikai
dc.contributor.authorSandler, Sarah E
dc.contributor.authorKeyser, Ulrich F
dc.date.accessioned2022-06-07T08:09:40Z
dc.date.available2022-06-07T08:09:40Z
dc.date.issued2022-04
dc.identifier.issn2589-0042
dc.identifier.other35479403
dc.identifier.otherPMC9036133
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/337721
dc.description.abstractNanopore sensing is an emerging technology that has many biosensing applications ranging from DNA sequencing using biological pores to biomolecular analysis using solid-state pores. Solid-state nanopores that are more stable are an attractive choice for biosensing applications. Still, biomolecule interactions with the nanopore surface reduce nanopore stability and increase usage costs. In this study, we investigated the biosensing capability for 102 quartz glass nanopores with a diameter of 11-18 nm that were fabricated using laser-assisted capillary pulling. Nanopores were assembled into multiple microfluidic chips that were repeatedly used for up to 19 weeks. We find that using vacuum storage combined with minimal washing steps improved the number of use cycles for nanopores. The single-molecule biosensing capability over repeated use cycles was demonstrated by quantitative analysis of a DNA carrier designed for detection of short single-stranded DNA oligonucleotides.
dc.languageeng
dc.publisherElsevier BV
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourcenlmid: 101724038
dc.sourceessn: 2589-0042
dc.subjectBiotechnology
dc.subjectNanotechnology
dc.subjectPhysical Chemistry
dc.subjectBiophysical Chemistry
dc.titleLifetime of glass nanopores in a PDMS chip for single-molecule sensing
dc.typeArticle
dc.date.updated2022-06-07T08:09:40Z
prism.issueIdentifier5
prism.publicationNameiScience
prism.volume25
dc.identifier.doi10.17863/CAM.85130
dcterms.dateAccepted2022-03-30
rioxxterms.versionofrecord10.1016/j.isci.2022.104191
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.contributor.orcidAlawami, Mohammed [0000-0003-2741-5436]
dc.contributor.orcidBoskovic, Filip [0000-0001-7663-2408]
dc.contributor.orcidZhu, Jinbo [0000-0003-4935-825X]
dc.contributor.orcidKeyser, Ulrich [0000-0003-3188-5414]
dc.identifier.eissn2589-0042
pubs.funder-project-idEngineering and Physical Sciences Research Council (2258738)
pubs.funder-project-idEngineering and Physical Sciences Research Council (EP/M008258/1)
pubs.funder-project-idEuropean Research Council (647144)
pubs.funder-project-idEngineering and Physical Sciences Research Council (EP/S022953/1)
pubs.funder-project-idEPSRC (EP/S023046/1)
cam.issuedOnline2022-04-04


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Attribution-NonCommercial-NoDerivatives 4.0 International
Except where otherwise noted, this item's licence is described as Attribution-NonCommercial-NoDerivatives 4.0 International