Broadening substrate specificity of a chain-extending ketosynthase through a single active-site mutation.


Change log
Authors
Murphy, Annabel C 
Hong, Hui 
Vance, Steve 
Broadhurst, R William 
Leadlay, Peter F 
Abstract

An in vitro model system based on a ketosynthase domain of the erythromycin polyketide synthase was used to probe the apparent substrate tolerance of ketosynthase domains of the mycolactone polyketide synthase. A specific residue change was identified that led to an emphatic increase in turnover of a range of substrates.

Description
Keywords
Catalytic Domain, Mutation, Polyketide Synthases, Protein Engineering, Substrate Specificity
Journal Title
Chem Commun (Camb)
Conference Name
Journal ISSN
1359-7345
1364-548X
Volume Title
52
Publisher
Royal Society of Chemistry (RSC)
Sponsorship
Wellcome Trust (094252/Z/10/Z)
BBSRC (BB/J007250/1)