CHCHD4 regulates the expression of mitochondrial genes that are essential for tumour cell growth
Accepted version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Abstract
CHCHD4 (MIA40) is central to the functions of the mitochondrial disulfide relay system (DRS). CHCHD4 is essential and evolutionarily conserved. Previously, we have shown CHCHD4 to be a critical regulator of tumour cell growth. Here, we use integrated analysis of our genome-wide CRISPR/Cas9 and SILAC proteomic screening data to delineate mechanisms of CHCHD4 essentiality in cancer. We identify a shortlist of common essential genes/proteins regulated by CHCHD4, including subunits of complex I that are known DRS substrates, and genes/proteins involved in key metabolic pathways. Our study highlights a range of CHCHD4-regulated nuclear encoded mitochondrial genes/proteins essential tumour cell growth.
Description
Keywords
CHCHD4, CRISPR/Cas9 genome-wide deletion screen, Gene essentiality, Mitochondria, Proteomics, SILAC analysis, Humans, Neoplasms, Mitochondria, Gene Expression Regulation, Neoplastic, Cell Proliferation, Mitochondrial Proteins, Mitochondrial Precursor Protein Import Complex Proteins, Genes, Mitochondrial, Cell Line, Tumor, Proteomics, CRISPR-Cas Systems, Mitochondrial Membrane Transport Proteins
Journal Title
Biochimica et Biophysica Acta - Molecular Basis of Disease
Conference Name
Journal ISSN
0925-4439
1879-260X
1879-260X
Volume Title
Publisher
Elsevier
Publisher DOI
Rights and licensing
Except where otherwised noted, this item's license is described as Attribution 4.0 International
Sponsorship
Medical Research Council (MR/K002201/2)
Cancer Research UK (19442)
Cancer Research UK (19442)
MRC, CR-UK, Wellcome Trust, Addenbrooke's Charitable Trust