The chinese herbal decoction danggui buxue tang inhibits angiogenesis in a rat model of liver fibrosis.


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Authors
Lv, Jing 
Zhao, Zhimin 
Chen, Yuan 
Wang, Qinglan 
Tao, Yanyan 
Abstract

In this study, we investigated the anti-angiogenic effect of the Chinese herbal decoction Danggui Buxue Tang (DBT; Radix Astragali and Radix Angelicae sinensis in 5 : 1 ratio) in a rat model of liver fibrosis, in order to elucidate its mechanisms of action against liver fibrosis. Liver fibrosis was induced with CCl(4) and high-fat food for 6 weeks, and the rats were treated with oral doses of DBT (6 g raw herbs/kg/d) and N-Acetyl-L-cysteine (NAC; 0.1 g/kg/d). The results showed that both DBT and NAC attenuated liver fibrosis and neo-angiogenesis. Furthermore, DBT and NAC improved SOD activity but decreased MDA content and 8-OH-dG in fibrotic livers, with DBT being more effective than NAC. DBT decreased the expression of VEGF, Ang1 and TGF-β1 and their signaling mediators, whereas NAC had no effect on VEGF and VEGFR2 expression. Both DBT and NAC reduced HIF-1α gene and protein expression in fibrotic livers, with DBT being more effective. These data clearly demonstrate that the anti-fibrotic properties of DBT are related to its ability to inhibit angiogenesis and its anti-angiogenic mechanisms are associated with improving oxidative stress, regulating the expression and signaling of angiogenic factors, and especially modulating HIF-1α in fibrotic livers.

Description
Keywords
1115 Pharmacology and Pharmaceutical Sciences, Biomedical, Basic Science, Complementary and Alternative Medicine, Chronic Liver Disease and Cirrhosis, Digestive Diseases, Liver Disease, Nutrition, Oral and Gastrointestinal
Journal Title
Evid Based Complement Alternat Med
Conference Name
Journal ISSN
1741-427X
1741-4288
Volume Title
Publisher
Hindawi Limited
Sponsorship
European Commission (223154)