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In Depth Exploration of the Alternative Proteome of Drosophila melanogaster.

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cam.issuedOnline2022-05-26
dc.contributor.authorFabre, Bertrand
dc.contributor.authorChoteau, Sebastien A
dc.contributor.authorDuboé, Carine
dc.contributor.authorPichereaux, Carole
dc.contributor.authorMontigny, Audrey
dc.contributor.authorKorona, Dagmara
dc.contributor.authorDeery, Michael
dc.contributor.authorCamus, Mylène
dc.contributor.authorBrun, Christine
dc.contributor.authorBurlet-Schiltz, Odile
dc.contributor.authorRussell, Steve
dc.contributor.authorCombier, Jean-Philippe
dc.contributor.authorLilley, Kathryn S
dc.contributor.authorPlaza, Serge
dc.contributor.orcidDeery, Michael [0000-0001-6895-9814]
dc.contributor.orcidRussell, Steve [0000-0003-0546-3031]
dc.date.accessioned2022-06-17T11:00:19Z
dc.date.available2022-06-17T11:00:19Z
dc.date.issued2022
dc.date.submitted2022-03-21
dc.date.updated2022-06-17T11:00:19Z
dc.description.abstractRecent studies have shown that hundreds of small proteins were occulted when protein-coding genes were annotated. These proteins, called alternative proteins, have failed to be annotated notably due to the short length of their open reading frame (less than 100 codons) or the enforced rule establishing that messenger RNAs (mRNAs) are monocistronic. Several alternative proteins were shown to be biologically active molecules and seem to be involved in a wide range of biological functions. However, genome-wide exploration of the alternative proteome is still limited to a few species. In the present article, we describe a deep peptidomics workflow which enabled the identification of 401 alternative proteins in Drosophila melanogaster. Subcellular localization, protein domains, and short linear motifs were predicted for 235 of the alternative proteins identified and point toward specific functions of these small proteins. Several alternative proteins had approximated abundances higher than their canonical counterparts, suggesting that these alternative proteins are actually the main products of their corresponding genes. Finally, we observed 14 alternative proteins with developmentally regulated expression patterns and 10 induced upon the heat-shock treatment of embryos, demonstrating stage or stress-specific production of alternative proteins.
dc.identifier.doi10.17863/CAM.85607
dc.identifier.eissn2296-634X
dc.identifier.issn2296-634X
dc.identifier.other901351
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/338196
dc.languageen
dc.publisherFrontiers Media SA
dc.subjectCell and Developmental Biology
dc.subjectalternative proteins
dc.subjectshort open reading frame–encoded polypeptide
dc.subjectmicroprotein
dc.subjectpeptidomics
dc.subjectmass spectrometry
dc.titleIn Depth Exploration of the Alternative Proteome of Drosophila melanogaster.
dc.typeArticle
dcterms.dateAccepted2022-04-25
prism.publicationNameFront Cell Dev Biol
prism.volume10
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/L002817/1)
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
rioxxterms.versionVoR
rioxxterms.versionofrecord10.3389/fcell.2022.901351

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