A shared role for sonic hedgehog signalling in patterning chondrichthyan gill arch appendages and tetrapod limbs.

Simple item page
cam.issuedOnline2016-04-19
dc.contributor.authorGillis, J Andrew
dc.contributor.authorHall, Brian K
dc.contributor.orcidGillis, James [0000-0003-2062-3777]
dc.date.accessioned2016-03-03T14:28:09Z
dc.date.available2016-03-03T14:28:09Z
dc.date.issued2016-04-15
dc.description.abstractChondrichthyans (sharks, skates, rays and holocephalans) possess paired appendages that project laterally from their gill arches, known as branchial rays. This led Carl Gegenbaur to propose that paired fins (and hence tetrapod limbs) originally evolved via transformation of gill arches. Tetrapod limbs are patterned by asonic hedgehog(Shh)-expressing signalling centre known as the zone of polarising activity, which establishes the anteroposterior axis of the limb bud and maintains proliferative expansion of limb endoskeletal progenitors. Here, we use loss-of-function, label-retention and fate-mapping approaches in the little skate to demonstrate that Shh secretion from a signalling centre in the developing gill arches establishes gill arch anteroposterior polarity and maintains the proliferative expansion of branchial ray endoskeletal progenitor cells. These findings highlight striking parallels in the axial patterning mechanisms employed by chondrichthyan branchial rays and paired fins/limbs, and provide mechanistic insight into the anatomical foundation of Gegenbaur's gill arch hypothesis.
dc.description.sponsorshipThis research was supported by a Royal Society University Research Fellowship [UF130182 to JAG], by Plum foundation John E. Dowling and Laura and Arthur Colwin Endowed Summer Research Fellowships at the Marine Biological Laboratory to JAG, by a grant from the University of Cambridge Isaac Newton Trust to [14.23z to JAG], and by a grant from the Natural Sciences and Engineering Research Council of Canada [A5056 to BKH].
dc.description.versionThis is the final version of the article. It first appeared from The Company of Biologists via http://dx.doi.org/10.1242/dev.133884
dc.identifier.eissn1477-9129
dc.identifier.issn0950-1991
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/254142
dc.languageEnglish
dc.language.isoen
dc.provenanceOA-7352
dc.publisherThe Company of Biologists
dc.publisher.urlhttp://dx.doi.org/10.1242/dev.133884
dc.subjectAppendage patterning
dc.subjectEvolution
dc.subjectGill arch
dc.subjectLeucoraja erinacea
dc.subjectSkate
dc.subjectSonic hedgehog
dc.subjectAnimals
dc.subjectExtremities
dc.subjectGene Expression
dc.subjectGills
dc.subjectHedgehog Proteins
dc.subjectLimb Buds
dc.subjectSignal Transduction
dc.subjectSkates, Fish
dc.titleA shared role for sonic hedgehog signalling in patterning chondrichthyan gill arch appendages and tetrapod limbs.
dc.typeArticle
dcterms.dateAccepted2016-02-22
prism.endingPage1317
prism.number8
prism.publicationDate2016
prism.publicationNameDevelopment
prism.startingPage1313
prism.volume143
rioxxterms.licenseref.startdate2016-04-19
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review
rioxxterms.versionVoR
rioxxterms.versionofrecord10.1242/dev.133884
Files
Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
Gillis_and_Hall-2016-Development-VoR.pdf
Size:
1002.52 KB
Format:
Adobe Portable Document Format
Description:
Published version
Licence
http://www.rioxx.net/licenses/all-rights-reserved
Download
Thumbnail Image
Name:
Gillis & Hall 2016 Development.pdf
Size:
7.41 MB
Format:
Adobe Portable Document Format
Description:
Accepted version
Licence
http://www.rioxx.net/licenses/all-rights-reserved
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.8 KB
Format:
Item-specific license agreed upon to submission
Download
Collections
Scholarly Works - Zoology
Symplectic mapped items for data match