A shared role for sonic hedgehog signalling in patterning chondrichthyan gill arch appendages and tetrapod limbs.

Gillis, J Andrew; Hall, Brian K

doi:10.1242/dev.133884

A shared role for sonic hedgehog signalling in patterning chondrichthyan gill arch appendages and tetrapod limbs.

cam.issuedOnline	2016-04-19
dc.contributor.author	Gillis, J Andrew
dc.contributor.author	Hall, Brian K
dc.contributor.orcid	Gillis, James [0000-0003-2062-3777]
dc.date.accessioned	2016-03-03T14:28:09Z
dc.date.available	2016-03-03T14:28:09Z
dc.date.issued	2016-04-15
dc.description.abstract	Chondrichthyans (sharks, skates, rays and holocephalans) possess paired appendages that project laterally from their gill arches, known as branchial rays. This led Carl Gegenbaur to propose that paired fins (and hence tetrapod limbs) originally evolved via transformation of gill arches. Tetrapod limbs are patterned by asonic hedgehog(Shh)-expressing signalling centre known as the zone of polarising activity, which establishes the anteroposterior axis of the limb bud and maintains proliferative expansion of limb endoskeletal progenitors. Here, we use loss-of-function, label-retention and fate-mapping approaches in the little skate to demonstrate that Shh secretion from a signalling centre in the developing gill arches establishes gill arch anteroposterior polarity and maintains the proliferative expansion of branchial ray endoskeletal progenitor cells. These findings highlight striking parallels in the axial patterning mechanisms employed by chondrichthyan branchial rays and paired fins/limbs, and provide mechanistic insight into the anatomical foundation of Gegenbaur's gill arch hypothesis.
dc.description.sponsorship	This research was supported by a Royal Society University Research Fellowship [UF130182 to JAG], by Plum foundation John E. Dowling and Laura and Arthur Colwin Endowed Summer Research Fellowships at the Marine Biological Laboratory to JAG, by a grant from the University of Cambridge Isaac Newton Trust to [14.23z to JAG], and by a grant from the Natural Sciences and Engineering Research Council of Canada [A5056 to BKH].
dc.description.version	This is the final version of the article. It first appeared from The Company of Biologists via http://dx.doi.org/10.1242/dev.133884
dc.identifier.eissn	1477-9129
dc.identifier.issn	0950-1991
dc.identifier.uri	https://www.repository.cam.ac.uk/handle/1810/254142
dc.language	English
dc.language.iso	eng
dc.provenance	OA-7352
dc.publisher	The Company of Biologists
dc.publisher.url	http://dx.doi.org/10.1242/dev.133884
dc.subject	Appendage patterning
dc.subject	Evolution
dc.subject	Gill arch
dc.subject	Leucoraja erinacea
dc.subject	Skate
dc.subject	Sonic hedgehog
dc.subject	Animals
dc.subject	Extremities
dc.subject	Gene Expression
dc.subject	Gills
dc.subject	Hedgehog Proteins
dc.subject	Limb Buds
dc.subject	Signal Transduction
dc.subject	Skates, Fish
dc.title	A shared role for sonic hedgehog signalling in patterning chondrichthyan gill arch appendages and tetrapod limbs.
dc.type	Article
dcterms.dateAccepted	2016-02-22
prism.endingPage	1317
prism.number	8
prism.publicationDate	2016
prism.publicationName	Development
prism.startingPage	1313
prism.volume	143
rioxxterms.licenseref.startdate	2016-04-19
rioxxterms.licenseref.uri	http://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.type	Journal Article/Review
rioxxterms.version	VoR
rioxxterms.versionofrecord	10.1242/dev.133884