Heavy Atom Detergent/Lipid Combined X-ray Crystallography for Elucidating the Structure-Function Relationships of Membrane Proteins.


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Authors
Abstract

Membrane proteins reside in the lipid bilayer of biomembranes and the structure and function of these proteins are closely related to their interactions with lipid molecules. Structural analyses of interactions between membrane proteins and lipids or detergents that constitute biological or artificial model membranes are important for understanding the functions and physicochemical properties of membrane proteins and biomembranes. Determination of membrane protein structures is much more difficult when compared with that of soluble proteins, but the development of various new technologies has accelerated the elucidation of the structure-function relationship of membrane proteins. This review summarizes the development of heavy atom derivative detergents and lipids that can be used for structural analysis of membrane proteins and their interactions with detergents/lipids, including their application with X-ray free-electron laser crystallography.

Description
Keywords
X-ray free-electron laser (XFEL), chemical synthesis, detergent, lipid, membrane protein, serial femtosecond crystallography (SFX), structure-function relationship
Journal Title
Membranes (Basel)
Conference Name
Journal ISSN
2077-0375
2077-0375
Volume Title
11
Publisher
MDPI AG
Sponsorship
Japan Science and Technology Agency (JPMJPR17GB, The ERATO active lipid structure project)
Ministry of Education, Culture, Sports, Science and Technology (19H05780, The X-ray Free Electron Laser Priority Strategy Program)
Japan Society for the Promotion of Science (16H06315)