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Reaction-Diffusion Patterning of DNA-Based Artificial Cells.

Published version
Peer-reviewed

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Authors

Leathers, Adrian 
Al Samad, Assala 
Kotar, Jurij 

Abstract

Biological cells display complex internal architectures with distinct micro environments that establish the chemical heterogeneity needed to sustain cellular functions. The continued efforts to create advanced cell mimics, namely, artificial cells, demands strategies for constructing similarly heterogeneous structures with localized functionalities. Here, we introduce a platform for constructing membraneless artificial cells from the self-assembly of synthetic DNA nanostructures in which internal domains can be established thanks to prescribed reaction-diffusion waves. The method, rationalized through numerical modeling, enables the formation of up to five distinct concentric environments in which functional moieties can be localized. As a proof-of-concept, we apply this platform to build DNA-based artificial cells in which a prototypical nucleus synthesizes fluorescent RNA aptamers that then accumulate in a surrounding storage shell, thus demonstrating the spatial segregation of functionalities reminiscent of that observed in biological cells.

Description

Keywords

Aptamers, Nucleotide, Artificial Cells, DNA, Diffusion, Nanostructures

Journal Title

J Am Chem Soc

Conference Name

Journal ISSN

0002-7863
1520-5126

Volume Title

144

Publisher

American Chemical Society (ACS)
Sponsorship
H2020 European Research Council (851667)
Engineering and Physical Sciences Research Council (EP/V030434/1)
Royal Society (RGF/EA/181009)
Royal Society (RGF/R1/180043)
Royal Society (UF160152)
Royal Society (URF/R1/180172)
University of Cambridge (NA)
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