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An artificial CO-releasing metalloprotein built by histidine-selective metallation.

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https://www.repository.cam.ac.uk/handle/1810/247467

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Albuquerque et al 2015 Chemical Communications.pdf (1.87 MB)

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Article

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Abstract

We report the design and synthesis of an aquacarbonyl Ru(II) dication cis-Ru(CO)2(H2O)4 reagent for histidine (His)-selective metallation of interleukin (IL)-8 at site 33. The artificial, non-toxic interleukin (IL)-8-Ru(II)(CO)2 metalloprotein retained IL-8-dependent neutrophil chemotactic activity and was shown to spontaneously release CO in live cells.

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Keywords

Biomimetic Materials, Chemotaxis, HeLa Cells, Histidine, Humans, Interleukin-8, Metalloproteins, Neutrophils, Ruthenium

Journal Title

Chem Commun (Camb)

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Journal ISSN

1359-7345
1364-548X

Volume Title

51

Publisher

Royal Society of Chemistry (RSC)

Publisher DOI

https://doi.org/10.1039/c4cc10204e

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Attribution 2.0 UK: England & Wales
Except where otherwised noted, this item's license is described as Attribution 2.0 UK: England & Wales
Sponsorship
Engineering and Physical Sciences Research Council (EP/M003647/1)
MRC (MC_PC_14116 v2)
We thank the European Commission (Marie Curie CIG to G.J.L.B., Marie Curie IEF to O.B.), FCT Portugal (FCT Investigator to G.J.L.B.) and the EPSRC for generous funding.

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Scholarly Works - Chemistry
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