Axon degeneration assays in Superior Cervical Ganglion explant cultures
Published version
Peer-reviewed
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Change log
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Abstract
The ability of peripheral nervous system neurons to extend long, axon-like neurites in vitro makes them ideally suited for studies on mechanisms of axon survival and degeneration. In this chapter we describe how to prepare explant cultures of sympathetic neurons of the superior cervical ganglion (SCG). We also describe how to induce and assess axon degeneration with an injury or a chemical insult.
Description
Keywords
Axon degeneration, Axon transection, Injury, SCG explant culture, Superior cervical ganglion, Wallerian degeneration, Animals, Antineoplastic Agents, Axons, Axotomy, Dissection, Mice, Microscopy, Phase-Contrast, Nerve Degeneration, Neurotoxins, Organ Culture Techniques, Protein Synthesis Inhibitors, Sensory Receptor Cells, Superior Cervical Ganglion, Wallerian Degeneration
Journal Title
Methods in Molecular Biology
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Journal ISSN
1064-3745
1940-6029
1940-6029
Volume Title
Publisher
Springer Nature
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Except where otherwised noted, this item's license is described as Attribution 4.0 International (CC BY)
Sponsorship
Medical Research Council (MR/N004582/1)
Wellcome Trust (210904/Z/18/Z)
Biotechnology and Biological Sciences Research Council (BB/S009582/1)
Wellcome Trust (210904/Z/18/Z)
Biotechnology and Biological Sciences Research Council (BB/S009582/1)