Visualization of individual cell division history in complex tissues using iCOUNT.

Change log
Denoth-Lippuner, Annina 
Jaeger, Baptiste N 
Liang, Tong 
Royall, Lars N 
Chie, Stefanie E 

The division potential of individual stem cells and the molecular consequences of successive rounds of proliferation remain largely unknown. Here, we developed an inducible cell division counter (iCOUNT) that reports cell division events in human and mouse tissues in vitro and in vivo. Analyzing cell division histories of neural stem/progenitor cells (NSPCs) in the developing and adult brain, we show that iCOUNT can provide novel insights into stem cell behavior. Further, we use single-cell RNA sequencing (scRNA-seq) of iCOUNT-labeled NSPCs and their progenies from the developing mouse cortex and forebrain-regionalized human organoids to identify functionally relevant molecular pathways that are commonly regulated between mouse and human cells, depending on individual cell division histories. Thus, we developed a tool to characterize the molecular consequences of repeated cell divisions of stem cells that allows an analysis of the cellular principles underlying tissue formation, homeostasis, and repair.

Cell division history, human brain organoid, imaging, neurogenesis, recombination, single cell RNA sequencing, stem cell proliferation, transgenesis, Animals, Brain, Cell Division, Cell Proliferation, Mice, Neural Stem Cells, Organoids, Sequence Analysis, RNA
Journal Title
Cell Stem Cell
Conference Name
Journal ISSN
Volume Title
Elsevier BV
Royal Society (RP/R1/180165)
Wellcome Trust (219478/Z/19/Z)
Wellcome Trust (098357/Z/12/Z)
Medical Research Council (MC_PC_17230)
Royal Society