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The origin, deployment, and evolution of a plant-parasitic nematode effectorome.

Published version
Peer-reviewed

Repository DOI


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Authors

Molloy, Beth 
Shin, Dio S 
Long, Jonathan 
Pellegrin, Clement 
Senatori, Beatrice 

Abstract

Plant-parasitic nematodes constrain global food security. During parasitism, they secrete effectors into the host plant from two types of pharyngeal gland cells. These effectors elicit profound changes in host biology to suppress immunity and establish a unique feeding organ from which the nematode draws nutrition. Despite the importance of effectors in nematode parasitism, there has been no comprehensive identification and characterisation of the effector repertoire of any plant-parasitic nematode. To address this, we advance techniques for gland cell isolation and transcriptional analysis to define a stringent annotation of putative effectors for the cyst nematode Heterodera schachtii at three key life-stages. We define 717 effector gene loci: 269 "known" high-confidence homologs of plant-parasitic nematode effectors, and 448 "novel" effectors with high gland cell expression. In doing so we define the most comprehensive "effectorome" of a plant-parasitic nematode to date. Using this effector definition, we provide the first systems-level understanding of the origin, deployment and evolution of a plant-parasitic nematode effectorome. The robust identification of the effector repertoire of a plant-parasitic nematode will underpin our understanding of nematode pathology, and hence, inform strategies for crop protection.

Description

Acknowledgements: This publication contains work funded by the Iowa Agriculture and Home Economics Experiment Station, Ames, IA, supported by Hatch Act and State of Iowa funds. This work was performed using resources provided by the Cambridge Service for Data Driven Discovery (CSD3) operated by the University of Cambridge Research Computing Service (www.csd3.cam.ac.uk), provided by Dell EMC and Intel using Tier-2 funding from the Engineering and Physical Sciences Research Council (capital grant EP/T022159/1), and DiRAC funding from the Science and Technology Facilities Council (www.dirac.ac.uk).


Funder: British Society of Plant Pathology

Keywords

Animals, Plant Diseases, Host-Parasite Interactions, Tylenchoidea, Plants, Helminth Proteins, Nematoda

Journal Title

PLoS Pathog

Conference Name

Journal ISSN

1553-7366
1553-7374

Volume Title

20

Publisher

Public Library of Science (PLoS)
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/R011311/1)
Biotechnology and Biological Sciences Research Council (BB/S006397/1)
European Commission Horizon 2020 (H2020) Marie Sk?odowska-Curie actions (882941)
EPSRC (EP/T022159/1)
BBSRC (BB/X006352/1)