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Super-Resolution Detection of DNA Nanostructures Using a Nanopore.

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Choudhary, Adnan 
Maffeo, Christopher  ORCID logo


High-resolution analysis of biomolecules has brought unprecedented insights into fundamental biological processes and dramatically advanced biosensing. Notwithstanding the ongoing resolution revolution in electron microscopy and optical imaging, only a few methods are presently available for high-resolution analysis of unlabeled single molecules in their native states. Here, label-free electrical sensing of structured single molecules with a spatial resolution down to single-digit nanometers is demonstrated. Using a narrow solid-state nanopore, the passage of a series of nanostructures attached to a freely translocating DNA molecule is detected, resolving individual nanostructures placed as close as 6 nm apart and with a surface-to-surface gap distance of only 2 nm. Such super-resolution ability is attributed to the nanostructure-induced enhancement of the electric field at the tip of the nanopore. This work demonstrates a general approach to improving the resolution of single-molecule nanopore sensing and presents a critical advance towards label-free, high-resolution DNA sequence mapping, and digital information storage independent of molecular motors.



DNA nanostructures, biosensing, nanopores, single molecules, super-resolution, Nanopores, Nanostructures, DNA, Nanotechnology, Electricity, Information Storage and Retrieval, Biosensing Techniques

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Adv Mater

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European Research Council (647144)
Engineering and Physical Sciences Research Council (EP/S022953/1)
European Commission Horizon 2020 (H2020) ERC (899538)