Research data supporting "Golgi-localized STELLO proteins regulate the assembly and trafficking of cellulose synthase complexes in Arabidopsis"


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Authors
Nikolovski, Nino 
Dupree, Ray 
Brown, Steven P. 
Description

1D solid state NMR data for mature stems of stello cellulose synthesis mutant and wild type plants. MAS solid state NMR experiments were performed on a widebore Bruker (Karlsruhe, Germany) AVANCE III 850 MHz solid state NMR spectrometer operating at 20 T, corresponding to 1H and 13C Larmor frequencies of 850.2 and 213.8 MHz, respectively, using a 4 mm double-resonance MAS probe. Experiments were conducted at room temperature at a MAS frequency of 12.0 kHz ± 5 Hz. The 13C cross-polarization (CP) MAS spectra were acquired using a 1ms ramped contact time, a spectral window of 100 kHz (468 ppm), a recycle delay of 2 s and 28800 transients were co-added for each sample. The 1H 90° pulse duration was 3.4 s. Two-pulse phase-modulated (TPPM) decoupling was applied during acquisition at a 1H nutation frequency of 83 kHz58. The 13C chemical shift was determined using the carbonyl peak at 177.8 ppm of L-alanine as an external reference with respect to TMS. Two independent stem samples were analysed.


This research data supports “Golgi-localized STELLO proteins regulate the assembly and trafficking of cellulose synthase complexes in Arabidopsis” which will be published in “Nature communications”.

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Keywords
stello, solid state NMR, arabidopsis
Publisher
University of Cambridge
Sponsorship
This work was supported by the BBSRC [grant number BB/G016240/1].
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