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Lifetime of glass nanopores in a PDMS chip for single-molecule sensing

Accepted version
Peer-reviewed

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Article

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Authors

Bošković, Filip 
Chen, Kaikai 
Sandler, Sarah E 

Abstract

Nanopore sensing is an emerging technology that has many biosensing applications ranging from DNA sequencing using biological pores to biomolecular analysis using solid-state pores. Solid-state nanopores that are more stable are an attractive choice for biosensing applications. Still, biomolecule interactions with the nanopore surface reduce nanopore stability and increase usage costs. In this study, we investigated the biosensing capability for 102 quartz glass nanopores with a diameter of 11-18 nm that were fabricated using laser-assisted capillary pulling. Nanopores were assembled into multiple microfluidic chips that were repeatedly used for up to 19 weeks. We find that using vacuum storage combined with minimal washing steps improved the number of use cycles for nanopores. The single-molecule biosensing capability over repeated use cycles was demonstrated by quantitative analysis of a DNA carrier designed for detection of short single-stranded DNA oligonucleotides.

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Journal Title

iScience

Conference Name

Journal ISSN

2589-0042
2589-0042

Volume Title

Publisher

Elsevier BV
Sponsorship
Engineering and Physical Sciences Research Council (2258738)
Engineering and Physical Sciences Research Council (EP/M008258/1)
European Research Council (647144)
Engineering and Physical Sciences Research Council (EP/S022953/1)
EPSRC (EP/S023046/1)