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A Temporal Proteomic Map of Epstein-Barr Virus Lytic Replication in B Cells

Published version
Peer-reviewed

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Type

Article

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Authors

Ersing, I 
Wang, LW 
Ma, Y 

Abstract

Epstein-Barr virus (EBV) replication contributes to multiple human diseases, including infectious mononucleosis, nasopharyngeal carcinoma, B cell lymphomas, and oral hairy leukoplakia. We performed systematic quantitative analyses of temporal changes in host and EBV proteins during lytic replication to gain insights into virus-host interactions, using conditional Burkitt lymphoma models of type I and II EBV infection. We quantified profiles of >8,000 cellular and 69 EBV proteins, including >500 plasma membrane proteins, providing temporal views of the lytic B cell proteome and EBV virome. Our approach revealed EBV-induced remodeling of cell cycle, innate and adaptive immune pathways, including upregulation of the complement cascade and proteasomal degradation of the B cell receptor complex, conserved between EBV types I and II. Cross-comparison with proteomic analyses of human cytomegalovirus infection and of a Kaposi-sarcoma-associated herpesvirus immunoevasin identified host factors targeted by multiple herpesviruses. Our results provide an important resource for studies of EBV replication.

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Keywords

B cell receptor, Epstein-Barr virus, complement, herpesvirus, host-pathogen interaction, immune evasion, lytic replication, quantitative proteomics, tandem mass tag, viral evasion

Journal Title

Cell Reports

Conference Name

Journal ISSN

2211-1247
2211-1247

Volume Title

19

Publisher

Elsevier
Sponsorship
Wellcome Trust (108070/Z/15/Z)
Wellcome Trust (100140/Z/12/Z)