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Automated tissue dissociation for the establishment of human intestinal organoids

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Peer-reviewed

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https://www.repository.cam.ac.uk/handle/1810/385198

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Primary Published version (3.87 MB)
 Supporting information (2.06 MB)
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Type

Article

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Abstract

Human intestinal Organoids (HIOs) may be set up from primary human tissue following dissociation, release, and culture of intestinal LGR5 + stem cells. Here, we characterise the use of a semi-automated mechanical tissue dissociation technique, which demonstrates an expedited workflow and streamlined protocol, standardising key aspects of the process while consistently maintaining a high level of cultured organoid viability.

Description

Acknowledgements: W.J.Y. was supported by a Cytiva sponsored Ph.D. studentship. Processing of human tissues and generation of human IEOs was supported by Helmsley Charitable Trust, funded by The Leona M and Harry B Helmsley Charitable Trust grant (G118500).We gratefully acknowledge Dr. Thomas Dennison for his valuable assistance with organoid staining and visualisation.

Keywords

Automation, Human intestinal organoids, Tissue dissociation, Humans, Organoids, Intestines, Stem Cells, Intestinal Mucosa, Receptors, G-Protein-Coupled, Cell Culture Techniques, Automation

Journal Title

Scientific Reports

Conference Name

Journal ISSN

2045-2322
2045-2322

Volume Title

15

Publisher

Nature Portfolio

Publisher DOI

https://doi.org/10.1038/s41598-025-03905-9

Rights and licensing

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Except where otherwised noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/
Sponsorship
Helmsley Charitable Trust, funded by The Leona M and Harry B Helmsley Charitable Trust (G118500, G118500, G118500, G118500, G118500)
Cytiva Studentship (refG117597)

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