Collagen-hyaluronic acid scaffolds for adipose tissue engineering.

Change log
Davidenko, N 
Campbell, JJ 
Thian, ES 
Watson, CJ 
Cameron, RE 

Three-dimensional (3-D) in vitro models of the mammary gland require a scaffold matrix that supports the development of adipose stroma within a robust freely permeable matrix. 3-D porous collagen-hyaluronic acid (HA: 7.5% and 15%) scaffolds were produced by controlled freeze-drying technique and crosslinking with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride. All scaffolds displayed uniform, interconnected pore structure (total porosity approximately 85%). Physical and chemical analysis showed no signs of collagen denaturation during the formation process. The values of thermal characteristics indicated that crosslinking occurred and that its efficiency was enhanced by the presence of HA. Although the crosslinking reduced the swelling of the strut material in water, the collagen-HA matrix as a whole tended to swell more and show higher dissolution resistance than pure collagen samples. The compressive modulus and elastic collapse stress were higher for collagen-HA composites. All the scaffolds were shown to support the proliferation and differentiation 3T3-L1 preadipocytes while collagen-HA samples maintained a significantly increased proportion of cycling cells (Ki-67+). Furthermore, collagen-HA composites displayed significantly raised Adipsin gene expression with adipogenic culture supplementation for 8 days vs. control conditions. These results indicate that collagen-HA scaffolds may offer robust, freely permeable 3-D matrices that enhance mammary stromal tissue development in vitro.

3T3-L1 Cells, Adipogenesis, Adipose Tissue, Animals, Biocompatible Materials, Biomarkers, Cells, Cultured, Collagen, Extracellular Matrix, Humans, Hyaluronic Acid, Mammary Glands, Human, Materials Testing, Mice, Porosity, Tissue Engineering, Tissue Scaffolds, Water
Journal Title
Acta Biomater
Conference Name
Journal ISSN
Volume Title
Elsevier BV
Biotechnology and Biological Sciences Research Council (BB/F000871/1)
This was supported by the Biotechnology and Biological Sciences Research Council.