An adherent tissue-inspired hydrogel delivery vehicle utilised in primary human glioma models.


Type
Article
Change log
Authors
Rowland, Matthew J 
Parkins, Christopher C 
McAbee, Joseph H 
Kolb, Anna K 
Hein, Robert 
Abstract

A physical hydrogel cross-linked via the host-guest interactions of cucurbit[8]uril and utilised as an implantable drug-delivery vehicle for the brain is described herein. Constructed from hyaluronic acid, this hydrogel is biocompatible and has a high water content of 98%. The mechanical properties have been characterised by rheology and compared with the modulus of human brain tissue demonstrating the production of a soft material that can be moulded into the cavity it is implanted into following surgical resection. Furthermore, effective delivery of therapeutic compounds and antibodies to primary human glioblastoma cell lines is showcased by a variety of in vitro and ex vivo viability and immunocytochemistry based assays.

Description
Keywords
Blood-brain barrier, Drug-delivery, Glioma, Hyaluronic acid, Hydrogel, cucurbit[8]uril, Blood-Brain Barrier, Brain, Cell Line, Tumor, Cell Survival, Drug Delivery Systems, Glioma, Humans, Hyaluronic Acid, Hydrogels, Immunohistochemistry
Journal Title
Biomaterials
Conference Name
Journal ISSN
0142-9612
1878-5905
Volume Title
179
Publisher
Elsevier BV
Sponsorship
Brain Tumour Charity (495097)
EPSRC (1652502)
This work was supported by The Brain Tumour Charity (RG89672), the National Institute for Health Research Cambridge Biomedical Research Centre; the Higher Education Funding Council for England. We acknowledge the Human Research Tissue Bank and Biomedical Research Centre for the tissue being accessed through the Human Research Tissue Bank. The Human Research Tissue Bank is supported by the NIHR Cambridge Biomedical Research Centre. MJR thanks the University of Cambridge Chemical Biology and Molecular Medicine PhD Training Programme for funding. CCP is thankful for the support of the EPSRC and the Brain Tumour Charity for funding. JHM acknowledges the support of the Gates Cambridge Scholarship programme. AKK was supported by a studentship from the John and Lucille van Geest Foundation.