Analysis of the Localization of MEN Components by Live Cell Imaging Microscopy.
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Peer-reviewed
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Abstract
Mitotic exit is determined by multiple spatial and temporal cues from the spindle poles and the two compartments in a dividing yeast cell-the mother and the bud. These signals are ultimately integrated by the activation of the mitotic exit network (MEN) to promote persistent release of Cdc14 from the nucleolus. Live imaging analysis using fluorescent protein tags is invaluable to dissect this critical decision-making trigger. Here, we present protocols for routine yeast live cell microscopy applicable to this problem.
Description
Keywords
Cell population analysis, Digital image, Fluorescence microscopy, Fluorescent protein tags, Live cell imaging, Single cell analysis, Still imaging, Time lapse, Anaphase, Cell Cycle Proteins, Green Fluorescent Proteins, Image Processing, Computer-Assisted, Microscopy, Fluorescence, Mitosis, Optical Imaging, Protein Serine-Threonine Kinases, Protein Tyrosine Phosphatases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Single-Cell Analysis
Is Part Of
The Mitotic Exit Network
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Publisher
Springer Nature
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ISBN
9781493965007
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Except where otherwised noted, this item's license is described as All rights reserved
Sponsorship
Wellcome Trust