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A sensitive mass spectrometric assay for mitochondrial CoQ pool redox state in vivo.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Burger, Nils 
Logan, Angela 
Prime, Tracy A 
Mottahedin, Amin 
Caldwell, Stuart T 

Abstract

Coenzyme Q (CoQ) is an essential cofactor, primarily found in the mitochondrial inner membrane where it functions as an electron carrier in the respiratory chain, and as a lipophilic antioxidant. The redox state of the CoQ pool is the ratio of its oxidised (ubiquinone) and reduced (ubiquinol) forms, and is a key indicator of mitochondrial bioenergetic and antioxidant status. However, the role of CoQ redox state in vivo is poorly understood, because determining its value is technically challenging due to redox changes during isolation, extraction and analysis. To address these problems, we have developed a sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay that enables us to extract and analyse both the CoQ redox state and the magnitude of the CoQ pool with negligible changes to redox state from small amounts of tissue. This will enable the physiological and pathophysiological roles of the CoQ redox state to be investigated in vivo.

Description

Keywords

CoQ(10), CoQ(9), Coenzyme Q, Mass spectrometry, Mitochondria, Oxidative stress, Redox state, Chromatography, Liquid, Mitochondria, Oxidation-Reduction, Tandem Mass Spectrometry, Ubiquinone

Journal Title

Free Radic Biol Med

Conference Name

Journal ISSN

0891-5849
1873-4596

Volume Title

147

Publisher

Elsevier BV
Sponsorship
Medical Research Council (MC_U105663142)
Wellcome Trust (110159/Z/15/Z)