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Conservation of vaccine antigen sequences encoded by sequenced strains of Streptococcus equi subsp. equi.

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cam.issuedOnline2022-01-19
dc.contributor.authorFrosth, Sara
dc.contributor.authorMorris, Ellen Ruth A
dc.contributor.authorWilson, Hayley
dc.contributor.authorFrykberg, Lars
dc.contributor.authorJacobsson, Karin
dc.contributor.authorParkhill, Julian
dc.contributor.authorFlock, Jan-Ingmar
dc.contributor.authorWood, Tim
dc.contributor.authorGuss, Bengt
dc.contributor.authorAanensen, David M
dc.contributor.authorBoyle, Ashley G
dc.contributor.authorRiihimäki, Miia
dc.contributor.authorCohen, Noah D
dc.contributor.authorWaller, Andrew S
dc.contributor.orcidFrosth, Sara [0000-0002-4080-5183]
dc.contributor.orcidMorris, Ellen Ruth A [0000-0002-7957-4642]
dc.contributor.orcidWilson, Hayley [0000-0002-9914-2601]
dc.contributor.orcidParkhill, Julian [0000-0002-7069-5958]
dc.contributor.orcidGuss, Bengt [0000-0002-7044-0701]
dc.contributor.orcidAanensen, David M [0000-0001-6688-0854]
dc.contributor.orcidBoyle, Ashley G [0000-0003-4325-1005]
dc.contributor.orcidCohen, Noah D [0000-0002-0384-2903]
dc.contributor.orcidWaller, Andrew S [0000-0002-7111-9549]
dc.date.accessioned2022-01-28T16:35:59Z
dc.date.available2022-01-28T16:35:59Z
dc.date.issued2023-01
dc.date.submitted2021-09-06
dc.date.updated2022-01-28T16:35:58Z
dc.descriptionFunder: Department of Large Animal Clinical Sciences
dc.descriptionFunder: USDA‐NIFA AgriLife and the Link Equine Research Endowment
dc.descriptionFunder: Texas A&M University; Id: http://dx.doi.org/10.13039/100007904
dc.descriptionFunder: Formas
dc.descriptionFunder: Intervacc AB
dc.descriptionFunder: The Horse Trust
dc.descriptionFunder: SEIB
dc.descriptionFunder: Paul Mellon Foundation
dc.descriptionFunder: Alice Noakes Memorial Charitable Trust
dc.descriptionFunder: Ivo Trust
dc.descriptionFunder: Tattersalls
dc.descriptionFunder: The Elise Pilkington Charitable Trust
dc.descriptionFunder: The European Breeders Fund
dc.descriptionFunder: The Serth and Gates Charity
dc.descriptionFunder: Margaret Giffen Charitable Trust
dc.descriptionFunder: The Payne Gallwey Charitable Trust
dc.descriptionFunder: The Stafford Trust
dc.descriptionFunder: Marjorie Coote Animal Charity Trust
dc.descriptionFunder: Beryl Evetts and Robert Luff Animal Welfare Trust
dc.descriptionFunder: The Anne Duchess of Westminster’s Charitable Trust
dc.description.abstractBACKGROUND: Streptococcus equi subspecies equi (S equi) is the cause of Strangles, one of the most prevalent diseases of horses worldwide. Variation within the immunodominant SeM protein has been documented, but a new eight-component fusion protein vaccine, Strangvac, does not contain live S equi or SeM and conservation of the antigens it contains have not been reported. OBJECTIVE: To define the diversity of the eight Strangvac antigens across a diverse S equi population. STUDY DESIGN: Genomic description. METHODS: Antigen sequences from the genomes of 759 S equi isolates from 19 countries, recovered between 1955 and 2018, were analysed. Predicted amino acid sequences in the antigen fragments of SEQ0256(Eq5), SEQ0402(Eq8), SEQ0721(EAG), SEQ0855(SclF), SEQ0935(CNE), SEQ0999(IdeE), SEQ1817(SclI) and SEQ2101(SclC) in Strangvac and SeM were extracted from the 759 assembled genomes and compared. RESULTS: The predicted amino acid sequences of SclC, SclI and IdeE were identical across all 759 genomes. CNE was truncated in the genome of five (0.7%) isolates. SclF was absent from one genome and another encoded a single amino acid substitution. EAG was truncated in two genomes. Eq5 was truncated in four genomes and 123 genomes encoded a single amino acid substitution. Eq8 was truncated in three genomes, one genome encoded four amino acid substitutions and 398 genomes encoded a single amino acid substitution at the final amino acid of the Eq8 antigen fragment. Therefore, at least 1579 (99.9%) of 1580 amino acids in Strangvac were identical in 743 (97.9%) genomes, and all genomes encoded identical amino acid sequences for at least six of the eight Strangvac antigens. MAIN LIMITATIONS: Three hundred and seven (40.4%) isolates in this study were recovered from horses in the UK. CONCLUSIONS: The predicted amino acid sequences of antigens in Strangvac were highly conserved across this collection of S equi.
dc.identifier.doi10.17863/CAM.80589
dc.identifier.eissn2042-3306
dc.identifier.issn0425-1644
dc.identifier.otherevj13552
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/333166
dc.languageen
dc.language.isoeng
dc.publisherWiley
dc.publisher.urlhttp://dx.doi.org/10.1111/evj.13552
dc.subjectS equi
dc.subjectgenetic conservation
dc.subjecthorse
dc.subjectstrangles
dc.subjectvaccine antigens
dc.subjectHorses
dc.subjectAnimals
dc.subjectStreptococcus equi
dc.subjectHorse Diseases
dc.subjectStreptococcus
dc.subjectStreptococcal Infections
dc.titleConservation of vaccine antigen sequences encoded by sequenced strains of Streptococcus equi subsp. equi.
dc.typeArticle
dcterms.dateAccepted2021-12-30
prism.publicationNameEquine Vet J
pubs.funder-project-idPetplan Charitable Trust (S19‐741‐780)
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
rioxxterms.versionVoR
rioxxterms.versionofrecord10.1111/evj.13552

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