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Functional neurological restoration of amputated peripheral nerve using biohybrid regenerative bioelectronics.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Carnicer-Lombarte, Alejandro  ORCID logo  https://orcid.org/0000-0002-5650-4692
Kawan, Malak 
Hilton, Sam 

Abstract

The development of neural interfaces with superior biocompatibility and improved tissue integration is vital for treating and restoring neurological functions in the nervous system. A critical factor is to increase the resolution for mapping neuronal inputs onto implants. For this purpose, we have developed a new category of neural interface comprising induced pluripotent stem cell (iPSC)-derived myocytes as biological targets for peripheral nerve inputs that are grafted onto a flexible electrode arrays. We show long-term survival and functional integration of a biohybrid device carrying human iPSC-derived cells with the forearm nerve bundle of freely moving rats, following 4 weeks of implantation. By improving the tissue-electronics interface with an intermediate cell layer, we have demonstrated enhanced resolution and electrical recording in vivo as a first step toward restorative therapies using regenerative bioelectronics.

Description

Keywords

Rats, Humans, Animals, Peripheral Nerves, Electrodes, Neurons, Nerve Regeneration

Journal Title

Sci Adv

Conference Name

Journal ISSN

2375-2548
2375-2548

Volume Title

9

Publisher

American Association for the Advancement of Science (AAAS)
Sponsorship
Engineering and Physical Sciences Research Council (EP/S009000/1)
European Commission Horizon 2020 (H2020) Future and Emerging Technologies (FET) (732032)
European Commission Horizon 2020 (H2020) Marie Sk?odowska-Curie actions (797506)
Sir Jules Thorn Charitable Trust (233838)
MRC (MC_UU_00014/5)