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Identification of Campylobacter jejuni and Campylobacter coli genes contributing to oxidative stress response using TraDIS analysis

Published version
Peer-reviewed

Repository DOI


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Authors

Stoakes, Emily 
Chen, Xuanlin 
Kalmar, Lajos 
Baker, Dave 
Evans, Rhiannon 

Abstract

jats:titleAbstract</jats:title>jats:sec jats:titleBackground</jats:title> jats:pjats:italicCampylobacter jejuni</jats:italic> and jats:italicCampylobacter coli</jats:italic> are the major causative agents of bacterial gastroenteritis worldwide and are known obligate microaerophiles. Despite being sensitive to oxygen and its reduction products, both species are readily isolated from animal food products kept under atmospheric conditions where they face high oxygen tension levels.</jats:p> </jats:sec>jats:sec jats:titleResults</jats:title> jats:pIn this study, Transposon Directed Insertion-site Sequencing (TraDIS) was used to investigate the ability of one jats:italicC. jejuni</jats:italic> strain and two jats:italicC. coli</jats:italic> strains to overcome oxidative stress, using Hjats:sub2</jats:sub>Ojats:sub2</jats:sub> to mimic oxidative stress. Genes were identified that were required for oxidative stress resistance for each individual strain but also allowed a comparison across the three strains. Mutations in the jats:italicperR</jats:italic> and jats:italicahpC</jats:italic> genes were found to increase jats:italicCampylobacter</jats:italic> tolerance to Hjats:sub2</jats:sub>Ojats:sub2</jats:sub>. The roles of these proteins in oxidative stress were previously known in jats:italicC. jejuni</jats:italic>, but this data indicates that they most likely play a similar role in jats:italicC. coli</jats:italic>. Mutation of jats:italicczcD</jats:italic> decreased jats:italicCampylobacter</jats:italic> tolerance to Hjats:sub2</jats:sub>Ojats:sub2</jats:sub>. The role of CzcD, which functions as a zinc exporter, has not previously been linked to oxidative stress. The TraDIS data was confirmed using defined deletions of jats:italicperR</jats:italic> and jats:italicczcD</jats:italic> in jats:italicC. coli</jats:italic> 15-537360.</jats:p> </jats:sec>jats:sec jats:titleConclusions</jats:title> jats:pThis is the first study to investigate gene fitness in both jats:italicC. jejuni</jats:italic> and jats:italicC. coli</jats:italic> under oxidative stress conditions and highlights both similar roles for certain genes for both species and highlights other genes that have a role under oxidative stress.</jats:p> </jats:sec>

Description

Acknowledgements: Not applicable.

Keywords

Oxidative stress, Campylobacter coli, Campylobacter jejuni, TraDIS, Aerobic stress

Journal Title

BMC Microbiology

Conference Name

Journal ISSN

1471-2180

Volume Title

24

Publisher

Springer Science and Business Media LLC
Sponsorship
Biotechnology and Biological Sciences Research Council (Institute Strategic Programme Microbes in the Food Chain [BB/R012504/1 and its constituent project BBS/E/F000PR10349 (Theme 2, Microbial Survival in the Food Chain])