Synthesis and biological evaluation of 1,2-disubsubstituted 4-quinolone analogues of Pseudonocardia sp. natural products.


Type
Article
Change log
Authors
Geddis, Stephen M 
Coroama, Teodora 
Forrest, Suzanne 
Hodgkinson, James T 
Abstract

A series of analogues of Pseudonocardia sp. natural products were synthesized, which have been reported to possess potent antibacterial activity against Helicobacter pylori and induce growth defects in Escherichia coli and Staphylococcus aureus. Taking inspiration from a methodology used in our total synthesis of natural products, we applied this methodology to access analogues possessing bulky N-substituents, traditionally considered to be challenging scaffolds. Screening of the library provided valuable insights into the structure-activity relationship of the bacterial growth defects, and suggested that selectivity between bacterial species should be attainable. Furthermore, a structurally related series of analogues was observed to inhibit production of the virulence factor pyocyanin in the human pathogen Pseudomonas aeruginosa, which may be a result of their similarity to the Pseudomonas quinolone signal (PQS) quorum sensing autoinducer. This provided new insights regarding the effect of N-substitution in PQS analogues, which has been hitherto underexplored.

Description
Keywords
antibiotics, cross-coupling, heterocycles, quorum-sensing, structure–activity relationships
Journal Title
Beilstein Journal of Organic Chemistry
Conference Name
Journal ISSN
1860-5397
1860-5397
Volume Title
14
Publisher
Beilstein-Institut
Sponsorship
Evelyn Trust (17/51)
Biotechnology and Biological Sciences Research Council (1804422)
Engineering and Physical Sciences Research Council (EP/P020291/1)
SF was supported by a BBSRC studentship. DRS acknowledges support from the Engineering and Physical Sciences Research Council (EP/P020291/1) and Royal Society (Wolfson Research Merit Award).