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Mechanisms for Maintaining Eukaryotic Replisome Progression in the Presence of DNA Damage.

Published version
Peer-reviewed

Change log

Authors

Guilliam, Thomas A 

Abstract

The eukaryotic replisome coordinates template unwinding and nascent-strand synthesis to drive DNA replication fork progression and complete efficient genome duplication. During its advancement along the parental template, each replisome may encounter an array of obstacles including damaged and structured DNA that impede its progression and threaten genome stability. A number of mechanisms exist to permit replisomes to overcome such obstacles, maintain their progression, and prevent fork collapse. A combination of recent advances in structural, biochemical, and single-molecule approaches have illuminated the architecture of the replisome during unperturbed replication, rationalised the impact of impediments to fork progression, and enhanced our understanding of DNA damage tolerance mechanisms and their regulation. This review focusses on these studies to provide an updated overview of the mechanisms that support replisomes to maintain their progression on an imperfect template.

Description

Keywords

DNA damage tolerance, DNA replication, recoupling, replication fork, replisome, repriming, translesion synthesis

Journal Title

Front Mol Biosci

Conference Name

Journal ISSN

2296-889X
2296-889X

Volume Title

8

Publisher

Frontiers Media SA