466. Immunogenicity of 2 nd, 3 rd, 4 th SARS-CoV-2 Vaccines in Renal Patient Populations

Abstract

Background: Dialysis, renal transplant (RTX) and autoimmune kidney disease (AKD) patients are at higher risk of COVD-19 and suboptimal humoral responses post primary SARS-CoV-2 vaccination. As such, these groups have been prioritised for further SARS-CoV-2 vaccine doses. Limited data are available on their immunogenicity. Methods: Patients on dialysis or with a RTX or AKD who received primary SARS-CoV-2 vaccination were prospectively recruited from three UK sites. SARS-CoV-2 IgG spike antibody (anti-S IgG) and nucleocapsid titres were measured with a Luminex assay post 2nd, 3rd and 4th SARS-CoV-2 vaccines. Endpoints were median anti-S IgG titres and seroconversion (anti-S IgG titre >1896 MFI). Results: 628 patients (58% male, median age 62 years) comprising 240 dialysis, 194 RTX, and 194 AKD patients were recruited, with seroconversion of 96%, 61% and 70% respectively post two vaccines, and median anti-S IgG titres (IQR) of 30604 (24393–31826), 5829 (455–28296) and 18711 (1233–30226). 400/627 (64%) had anti-S IgG titres post 3rd dose (82% BNT162b2, 7% mRNA-1273, 3% ChAdOx1, 8% unknown; median 191 days post 2nd dose), with 27/58 (47%) of RTX and 17/46 (37%) of AKD patient non-responders seroconverting. Seroconversion among RTX and AKD patients overall improved to 78% (114/146) and 79% (122/155) respectively (p< 0.05), but not in dialysis patients post 3rd dose. 212/400 (53%) had serology post 4th dose (66% BNT162b2, 22% mRNA-1273, 12% unknown; median 104 days post 3rd dose), with 10/22 (46%) and 3/25 (12%) of RTX and AKD patient non-responders seroconverting. Anti-S IgG titre and seroconversion were similar within each group post 4th dose (Table 1, Figure 1). Predictors of non-seroconversion post 3rd dose in a multivariable model were rituximab use within six months, mycophenolate, prednisolone, chronic kidney disease and AKD (all p< 0.05), and post 4th dose, rituximab alone (p< 0.001). Conclusion: After primary SARS-CoV-2 vaccination, a 3rd but not 4th dose improved anti-S IgG titres and seroconversion in renal patients. Immunosuppressive use, particularly rituximab, was associated with reduced serologic responses (Tables 2, 3). Patients on immunosuppressives should therefore be prioritised for consideration of additional pre-exposure prophylactic agents. Disclosures: Michael Chen-Xu, MBChB, MRCP, MPH, GSK: Grant/Research Support Rachel Jones, MD, GSK: Advisor/Consultant|GSK: Grant/Research Support|Roche: Grant/Research Support|Vifor Pharma: Advisor/Consultant Rona M. Smith, MD MRCP, GSK: Grant/Research Support|Union Therapeutics: Grant/Research Support