Repository logo
 

University of Cambridge Preprints Collection

The mission of the University of Cambridge is to contribute to society through the pursuit of education, learning, and research at the highest international levels of excellence. This collection contains the latest unpublished research outputs (working papers, preprints) of the University's academic staff and students.

Browse

Recent Submissions

Now showing 1 - 4 of 4
  • ItemOpen AccessPreprint
    Measurement and modelling of driver learning of steering control during successive obstacle avoidance manoeuvres
    (2024-05-30) Fieldhouse, Harry; Keen, Steven; Cole, David; Cole, David [0000-0003-3162-701X]
    Understanding of driver behaviour can provide invaluable insight for the design of vehicles and driver assistance systems. Most existing human driver models do not incorporate driver learning or the effect of a driver’s confidence in their predictions of future states, both of which affect human-generated control actions. In this paper, human driver steering control is assessed based on experimental data, then a driver model is proposed which captures driver learning and is capable of reproducing a wide range of human control styles by the selection of appropriate parameters. The driver model learns an internal model of the vehicle dynamics from experience, using a Gaussian Process, then selects control actions via Model Predictive Control. The results verify the model’s capacity to capture the learning drivers achieve over time and to replicate various observed cautious and adventurous behaviours.
  • ItemOpen Access
    Sociologists’ Futurity Formation Through Hauntology
    (2024-04-13) Peng, Sheng-Hsiang; Peng, Sheng-Hsiang [0000-0003-1825-6146]
    At its core, Sociology thrives when it casts its gaze outward, going into the knots of human society: its myriad groups, organisations, and institutions; the diverse environments individuals inhabit, whether natural or man-made; the intricate belief systems and social norms that shape daily existence; and the fluid dynamics of power and resistance that underpin societal frameworks and catalyse evolution.
  • ItemOpen AccessPreprint
    Highly Multiplexed Proteomic Analysis of HCMV Infected Dendritic Cells Reveals Global Manipulation of Adaptive Immunity and Host Restriction of Viral Replication
    Kerr-Jones, Lauren; Soday, Lior; Cwyfan Hughes, Nia; Xinyue, Wang; Leah, Hunter; Antrobus, Robin; Miners, Kelly; Ladell, Kristin; Price, David; Fielding, Ceri; Wang, Eddie; Weekes, Michael; Stanton, Richard; Weekes, Michael [0000-0003-3196-5545]
    HCMV is a clinically significant herpesvirus and a paradigm for pathogen-mediated immune-evasion. Its broad tropism includes antigen-presenting cells such as dendritic cells (DCs), which may partly explain a unique, dramatic imprint on host immunity that occurs following lifelong carriage. Despite this, most studies use fibroblasts as a model. We therefore developed systems to isolate pure populations of DCs following infection with wild-type HCMV, before applying our quantitative temporal proteomic technologies to systematically characterise the virus:DC interaction within cells and at the cell surface. This comprehensive dataset quantifying almost 9,000 proteins throughout the infection timecourse revealed multiple DC-specific viral:host effects, including key impacts on innate, intrinsic, and adaptive immunity. These included observations that APOBEC3A is downregulated in infected cells and restricts HCMV infection in ex vivo DCs, delaying the progression of lytic infection, and that cell surface ICOS-Ligand was downregulated by two viral genes, inhibiting the induction of adaptive immunity.
  • ItemOpen AccessPreprint
    Spatial proteomics identifies a novel CRTC-dependent viral sensing pathway that stimulates production of Interleukin-11
    Ravenhill, Benjamin; Oliveira, Marisa; Wood, George; Di, Ying; Davies, Colin; Lu, Yongxu; Antrobus, Robin; Elliott, Gill; Irigoyen, Nerea; Hughes, David; Lyons, Paul; Chung, Betty; Borner, Georg; Weekes, Michael; Weekes, Michael [0000-0003-3196-5545]
    Appropriate cellular recognition of viruses is essential for the generation of effective innate and adaptive antiviral immunity. Viral sensors and their signalling components thus provide a crucial first line of host defence. Many exhibit subcellular relocalisation upon activation, triggering expression of interferon and antiviral genes. To identify novel signalling factors we analysed protein relocalisation on a global scale during viral infection. CREB Regulated Transcription Coactivators-2 and 3 (CRTC2/3) exhibited early cytoplasmic-to-nuclear translocation upon a diversity of viral stimuli, in diverse cell types. This movement was depended on Mitochondrial Antiviral Signalling Protein (MAVS), cyclo-oxygenase proteins and protein kinase A. We identify a key effect of transcription stimulated by CRTC2/3 translocation as production of the pro-fibrogenic cytokine interleukin-11. This may be important clinically in viral infections associated with fibrosis, including SARS-CoV-2.