Regulation of chemotropic guidance of nerve growth cones by microRNA
Lynn, Rachel C
Bassell, Gary J
Zheng, James Q
MetadataShow full item record
Han, L., Wen, Z., Lynn, R. C., Baudet, M., Holt, C. E., Sasaki, Y., Bassell, G. J., & et al. (2011). Regulation of chemotropic guidance of nerve growth cones by microRNA.
Abstract Background The small non-coding microRNAs play an important role in development by regulating protein translation, but their involvement in axon guidance is unknown. Here, we investigated the role of microRNA-134 (miR-134) in chemotropic guidance of nerve growth cones. Results We found that miR-134 is highly expressed in the neural tube of Xenopus embryos. Fluorescent in situ hybridization also showed that miR-134 is enriched in the growth cones of Xenopus spinal neurons in culture. Importantly, overexpression of miR-134 mimics or antisense inhibitors blocked protein synthesis (PS)-dependent attractive responses of Xenopus growth cones to a gradient of brain-derived neurotrophic factor (BDNF). However, miR-134 mimics or inhibitors had no effect on PS-independent bidirectional responses of Xenopus growth cones to bone morphogenic protein 7 (BMP7). Our data further showed that Xenopus LIM kinase 1 (Xlimk1) mRNA is a potential target of miR-134 regulation. Conclusions These findings demonstrate a role for miR-134 in translation-dependent guidance of nerve growth cones. Different guidance cues may act through distinct signaling pathways to elicit PS-dependent and -independent mechanisms to steer growth cones in response to a wide array of spatiotemporal cues during development.
This record's URL: http://www.dspace.cam.ac.uk/handle/1810/240573
Rights Holder: Han et al.; licensee BioMed Central Ltd.