Lipidomic analyses, breast- and formula-feeding, and growth in infancy
Journal of Pediatrics
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Prentice, P., Koulman, A., Matthews, L., Acerini, C., Ong, K., & Dunger, D. (2014). Lipidomic analyses, breast- and formula-feeding, and growth in infancy. Journal of Pediatrics, 166 276-281.e6. https://doi.org/10.1016/j.jpeds.2014.10.021
Objective: Suggested benefits of breast-feeding during infancy, including reduced risk of obesity and improved neurodevelopmental outcomes, remain unexplained but could be mediated by or reflected in lipidomic profiles. We evaluated lipidomic differences between breast- and formula-fed infants. Study design: We utilised high-resolution mass-spectrometry methods to analyse 3.2 mm dried blood spot samples (DBS) collected at ages 3 months (n=241) and 12 months (n=144) from a representative birth cohort study. Lipidomic profiles were compared between infants exclusively breast-fed, formula-fed or mixed-fed, and related to 12-month infancy weight. Data analysis included supervised multivariate statistics (PLS-DA) and univariate analysis with correction for multiple testing. Results: Distinct differences in 3-month lipidomic profiles were observed between exclusively breast-fed and formula-fed infants; mixed-fed infants showed intermediate profiles. Principal lipidomic characteristics of breast-fed infants were: lower total phosphatidylcholines (PC), with specifically lower short chain unsaturated PC but higher long chain polyunsaturated PC; higher cholesterol esters; variable differences in sphingomyelins. At 12 months, lipidomic profiles were markedly different to those at 3 months, and differences between the earlier breast/formula/mixed-feeding groups were no longer evident. However, several specific lipid species, associated with breast-feeding at 3 months, also correlated with differences in 3-12 month weight. Conclusion: State-of-the-art DBS lipidomic profiling demonstrated striking differences between breast-fed and formula-fed infants. Although these changes diminished with age, breast-fed lipidomic profiles at 3 months were associated with infancy weight and could potentially represent biomarkers of infant nutrition.
PP was supported by a UK MRC Clinical Training Fellowship (G1001995). The Cambridge Baby Growth Study has been supported by the European Union, the World Cancer Research Foundation International, the Medical Research Council (including a centenary award), and the NIHR Cambridge Comprehensive Biomedical Research Centre. The lipidomics assays were supported by the Medical Research Council (UD99999906 and Cambridge Lipidomics Biomarker Research Initiative G0800783).
Cambridge University Hospitals NHS Foundation Trust (CUH) (unknown)
Biotechnology and Biological Sciences Research Council (BB/M027252/1)
Medical Research Council (MC_U106179472)
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External DOI: https://doi.org/10.1016/j.jpeds.2014.10.021
This record's URL: https://www.repository.cam.ac.uk/handle/1810/246144
Attribution 2.0 UK: England & Wales
Licence URL: http://creativecommons.org/licenses/by/2.0/uk/