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dc.contributor.authorGraham, Sarah JLen
dc.contributor.authorWicher, Krzysztof Ben
dc.contributor.authorJedrusik, Agnieszkaen
dc.contributor.authorGuo, Guojien
dc.contributor.authorHerath, Wishvaen
dc.contributor.authorRobson, Paulen
dc.contributor.authorZernicka-Goetz, Magdalenaen
dc.date.accessioned2014-10-20T11:30:22Z
dc.date.available2014-10-20T11:30:22Z
dc.date.issued2014en
dc.identifier.citationGraham et al. Nature Communications (2014) Vol. 5, Article number 5667. DOI: 10.1038/ncomms6667en
dc.identifier.issn2041-1723
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/246215
dc.description.abstractPre-implantation development requires the specification and organization of embryonic and extra-embryonic lineages. The separation of these lineages takes place when asymmetric divisions generate inside and outside cells that differ in polarity, position and fate. Here we assess the global transcriptional identities of these precursor cells to gain insight into the molecular mechanisms regulating lineage segregation,. Unexpectedly, this reveals that complementary components of the BMP signalling pathway are already differentially expressed after the first wave of asymmetric divisions. We investigate the role of BMP signalling by expressing dominant negative forms of Smad4 and Bmpr2, by down-regulating the pathway using RNAi against BMP ligands and by applying three different BMP inhibitors at distinct stages. This reveals that BMP signalling regulates the correct development of both extra-embryonic lineages, primitive endoderm and trophectoderm, but not the embryonic lineage, prior to implantation. Together these findings indicate multiple roles of BMP signalling in the early mouse embryo.
dc.description.sponsorshipThis work was supported by a Wellcome Trust programme grant to MZG and an Agency for Science, Technology and Research (A*Star) core research budget to PR.
dc.languageEnglishen
dc.language.isoenen
dc.publisherNature Publishing Group
dc.subjectembryoen
dc.subjectmouseen
dc.subjectBMP signallingen
dc.subjectdevelopmenten
dc.subjectpluripotencyen
dc.subjectdifferentiationen
dc.titleBMP signalling regulates the pre-implantation development of extra-embryonic cell lineages in the mouse embryoen
dc.typeArticle
dc.description.versionThis is the author accepted manuscript. The final version is available from NPG via http://dx.doi.org/10.1038/ncomms6667en
prism.numberArticle number 5667en
prism.publicationDate2014en
prism.publicationNameNature Communicationsen
prism.volume5en
dc.rioxxterms.funderWellcome Trust
dcterms.dateAccepted2014-10-24en
rioxxterms.versionofrecord10.1038/ncomms6667en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2014en
dc.contributor.orcidZernicka-Goetz, Magdalena [0000-0002-7004-2471]
dc.identifier.eissn2041-1723
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWellcome Trust (098287/Z/12/Z)


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