Estimation of the spontaneous mutation rate in Heliconius melpomene
Keightley, Peter D
Ness, Rob W
Dasmahapatra, Kanchon K
Molecular Biology and Evolution
Oxford Journals on behalf of the Society for Molecular Biology and Evolution
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Keightley, P. D., Pinharanda, A., Ness, R. W., Simpson, F., Dasmahapatra, K. K., Mallet, J., Davey, J., & et al. (2014). Estimation of the spontaneous mutation rate in Heliconius melpomene. Molecular Biology and Evolution, 32 239-243. https://doi.org/10.1093/molbev/msu302
We estimated the spontaneous mutation rate in Heliconius melpomene by genome sequencing of a pair of parents and 30 of their offspring, based on the ratio of number of de novo heterozygotes to the number of callable site-individuals. We detected nine new mutations, each one affecting a single site in a single offspring. This yields an estimated mutation rate of 2.9 x 10-9 (95% confidence interval, 1.3 x 10-9 - 5.5 x 10-9), which is similar to recent estimates in Drosophila melanogaster, the only other insect species in which the mutation rate has been directly estimated. We infer that recent effective population size of H. melpomene is about 2 million, a substantially lower value than its census size, suggesting a role for natural selection reducing diversity. We estimate that H. melpomene diverged from its Müllerian co-mimic H. erato about 6 MYA, a somewhat later date than estimates based on a local molecular clock.
CJ was funded by BBSRC [H01439X/1], JWD was funded by the Herchel Smith Fund and PDK and RWN were funded by the BBSRC.
External DOI: https://doi.org/10.1093/molbev/msu302
This record's URL: https://www.repository.cam.ac.uk/handle/1810/246376
Attribution 2.0 UK: England & Wales
Licence URL: http://creativecommons.org/licenses/by/2.0/uk/
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