G-quadruplex ligands exhibit differential G-tetrad selectivity

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Authors
Le, Daniel
Di, Antonio M
Chan, Louis
  Balasubramanian, Shankar
Publication Date
2015-04-03
Journal Title
Chemical Communications
ISSN
1359-7345
Publisher
RSC
Volume
51
Pages
8048-8050
Language
English
Type
Article
Metadata
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Citation
Le, D., Di, A. M., Chan, L., & Balasubramanian, S. (2015). G-quadruplex ligands exhibit differential G-tetrad selectivity. Chemical Communications, 51 8048-8050. https://doi.org/10.1039/C5CC02252E
Abstract
A rapid and simple equilibrium-binding assay mediated by ligand-induced fluorescence quenching of fluorophore-labelled G-quadruplex (G4) structures enabled quantitative interrogation of mutually exclusive ligand binding interactions at opposed G-tetrads. This technique revealed that the ligands TmPyP4, PhenDC3, and PDS have differential chemotype-specific binding preferences for individual G-tetrads of a model genomic G4 structure.
Sponsorship
This work was supported by the following grants: Cancer Research UK Programme, BBSRC BB/K018043/1 and EPSRC EP/ K039520/1. We thank Dr Chris Lowe for his constructive comments and for proofreading the manuscript.
Funder references
BBSRC (BB/K018043/1)
EPSRC (EP/K039520/1)
Identifiers
External DOI: https://doi.org/10.1039/C5CC02252E
This record's URL: https://www.repository.cam.ac.uk/handle/1810/248056

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Attribution 2.0 UK: England & Wales
Licence URL: http://creativecommons.org/licenses/by/2.0/uk/


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Attribution 2.0 UK: England & Wales
Except where otherwise noted, this item's licence is described as Attribution 2.0 UK: England & Wales