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Full establishment of arbuscular mycorrhizal symbiosis in rice occurs independently of enzymatic jasmonate biosynthesis.


Type

Article

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Authors

Gutjahr, Caroline 
Siegler, Heike 
Haga, Ken 
Iino, Moritoshi 

Abstract

Development of the mutualistic arbuscular mycorrhiza (AM) symbiosis between most land plants and fungi of the Glomeromycota is regulated by phytohormones. The role of jasmonate (JA) in AM colonization has been investigated in the dicotyledons Medicago truncatula, tomato and Nicotiana attenuata and contradicting results have been obtained with respect to a neutral, promotive or inhibitory effect of JA on AM colonization. Furthermore, it is currently unknown whether JA plays a role in AM colonization of monocotyledonous roots. Therefore we examined whether JA biosynthesis is required for AM colonization of the monocot rice. To this end we employed the rice mutant constitutive photomorphogenesis 2 (cpm2), which is deficient in JA biosynthesis. Through a time course experiment the amount and morphology of fungal colonization did not differ between wild-type and cpm2 roots. Furthermore, no significant difference in the expression of AM marker genes was detected between wild type and cpm2. However, treatment of wild-type roots with 50 μM JA lead to a decrease of AM colonization and this was correlated with induction of the defense gene PR4. These results indicate that JA is not required for AM colonization of rice but high levels of JA in the roots suppress AM development likely through the induction of defense.

Description

Keywords

Cyclopentanes, Genes, Plant, Glomeromycota, Intramolecular Oxidoreductases, Mutation, Mycorrhizae, Oryza, Oxylipins, Plant Growth Regulators, Plant Proteins, Symbiosis

Journal Title

PLoS One

Conference Name

Journal ISSN

1932-6203
1932-6203

Volume Title

10

Publisher

Public Library of Science (PLoS)
Sponsorship
CG was supported by a PhD fellowship of the German National Merit Foundation (Studienstiftung des Deutschen Volkes); http://www.studienstiftung.de/ and the Roche Foundation (Switzerland). Research in the UP laboratory was supported by the SNF 'professeur boursier' grants PP00A-110874 and PP00P3-130704; http://www.snf.ch/en/Pages/default.aspx. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.