5-Formylcytosine can be a stable DNA modification in mammals
Burgess, Heather E
Nature Chemical Biology
Nature Publishing Group
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Bachman, M., Uribe-Lewis, S., Yang, X., Burgess, H. E., Iurlaro, M., Reik, W., Murrell, A., & et al. (2015). 5-Formylcytosine can be a stable DNA modification in mammals. Nature Chemical Biology, 11 555-557. https://www.repository.cam.ac.uk/handle/1810/248431
5-formylcytosine (5fC) is a rare base found in mammalian DNA and thought to be involved in active DNA demethylation. Here, we show that developmental dynamics of 5fC levels in mouse DNA differ from those of 5-hydroxymethylcytosine (5hmC), and using stable isotope labelling in vivo, we show that 5fC can be a stable DNA modification. These results suggest 5fC has functional roles in DNA that go beyond being a demethylation intermediate.
This work was supported by the Cancer Research UK (C14303/A17197, S.B.), The Wellcome Trust (WT099232, S.B.; WT095645/Z/11/Z, W.R.) and the BBSRC (BB/K010867/1, W.R.).
Wellcome Trust (099232/Z/12/Z)
This record's URL: https://www.repository.cam.ac.uk/handle/1810/248431