Structure and boosting activity of a starch-degrading lytic polysaccharide monooxygenase.

Lo Leggio, Leila 
Simmons, Thomas J 
Poulsen, Jens-Christian N 
Frandsen, Kristian EH 
Hemsworth, Glyn R 

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Lytic polysaccharide monooxygenases (LPMOs) are recently discovered enzymes that oxidatively deconstruct polysaccharides. LPMOs are fundamental in the effective utilization of these substrates by bacteria and fungi; moreover, the enzymes have significant industrial importance. We report here the activity, spectroscopy and three-dimensional structure of a starch-active LPMO, a representative of the new CAZy AA13 family. We demonstrate that these enzymes generate aldonic acid-terminated malto-oligosaccharides from retrograded starch and boost significantly the conversion of this recalcitrant substrate to maltose by β-amylase. The detailed structure of the enzyme's active site yields insights into the mechanism of action of this important class of enzymes.

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Acids, Catalytic Domain, Cellulose, Copper, Crystallography, X-Ray, Electron Spin Resonance Spectroscopy, Evolution, Molecular, Fungi, Genomics, Histidine, Maltose, Mixed Function Oxygenases, Oligosaccharides, Oxygen, Phylogeny, Polysaccharides, Protein Conformation, Protein Structure, Tertiary, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Starch, Substrate Specificity, beta-Amylase
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Nat Commun
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Springer Science and Business Media LLC
BBSRC (via University of York) (R1500502)
Biotechnology and Biological Sciences Research Council (BB/L000423/1)
This work was supported by a grant from the European Research Agency—Industrial Biotechnology Initiative as financed by the national research councils: Biotechnology and Biological Sciences Research Council (grant number BB/L000423) and Agence Française de l'Environnement et de la Maîtrise de l'Energie (grant number 1201C102). The Danish Council for Strategic Research (grant numbers 12-134923 and 12-134922). The Danish Ministry of Higher Education and Science through the Instrument Center DANSCATT and the European Community’s Seventh Framework Programme (FP7/2007-2013) under BioStruct-X (grant agreement N°283570) funded travel to synchrotrons. P.H.W. acknowledges the experimental assistance of Rebecca Gregory and Dr Victor Chechik. L.L.L. acknowledges the experimental assistance of Dorthe Boelskifte and the ESRF and MAXLAB staff for assistance with data collection.