Different Drosophila cell types exhibit differences in mitotic centrosome assembly dynamics
Raff, Jordan W
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Conduit, P., & Raff, J. W. (2015). Different Drosophila cell types exhibit differences in mitotic centrosome assembly dynamics. Current Biology, 25 R650-R651. https://doi.org/10.1016/j.cub.2015.05.061
Centrosomes are major microtubule organising centres comprising a pair of centrioles surrounded by pericentriolar material (PCM). The PCM expands dramatically as cells enter mitosis, and we previously showed that two key PCM components, Centrosomin (Cnn) and Spd-2, cooperate to form a scaffold structure around the centrioles that recruits the mitotic PCM in Drosophila; the SPD-5 and SPD-2 proteins appear to play a similar function in C. elegans 1, 2 and 3. In fly syncytial embryos, Cnn and Spd-2 are initially recruited into a central region of the PCM and then flux outwards 4, 5 and 6. This centrosomal flux is potentially important, but it has so far not been reported in any other cell type. Here we examine the dynamic behaviour of Cnn and Spd-2 in Drosophila larval brain cells. Spd-2 fluxes outwards from the centrioles in both brains and embryos in a microtubule-independent manner. In contrast, although Cnn is initially incorporated into the region of the PCM occupied by Spd-2 in both brains and embryos, Cnn fluxes outwards along microtubules in embryos, but not in brain cells, where it remains concentrated around the centrosomal Spd-2. Thus, the microtubule-independent centrosomal-flux of Spd-2 occurs in multiple fly cell types, while the microtubule-dependent outward flux of Cnn appears to be restricted to the syncytial embryo.
P.T.C. is supported by a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (105653/Z/14/Z). J.R. is supported by a Senior Investigator award funded by the Wellcome Trust (104575/Z/14/Z).
WELLCOME TRUST (105653/Z/14/Z)
External DOI: https://doi.org/10.1016/j.cub.2015.05.061
This record's URL: https://www.repository.cam.ac.uk/handle/1810/251095
Attribution 2.0 UK: England & Wales
Licence URL: http://creativecommons.org/licenses/by/2.0/uk/
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