Effect of pre-warming EDTA blood samples to 37oC on platelet count measured by Sysmex XT-2000iV in dogs, cats and horses
Effect of sample warming on platelet count
Veterinary Clinical Pathology
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Williams, T., & Archer, J. (2016). Effect of pre-warming EDTA blood samples to 37oC on platelet count measured by Sysmex XT-2000iV in dogs, cats and horses. Veterinary Clinical Pathology, 45 444-449. https://doi.org/10.1111/vcp.12378
Background: Pseudothrombocytopenia secondary to platelet clumping is a common cause of pre-analytical error for platelet counts in dogs, cats and horses. In humans, it is suggested that pre-warming blood samples to 37oC prior to haematology analysis will reduce platelet clumping. Objectives: To evaluate the effect of pre-warming EDTA blood samples to 37oC on measured platelet counts and other haematological parameters. Methods: EDTA blood samples from dogs, cats and horses submitted to the clinical pathology laboratory at the University of Cambridge were included. Complete blood counts (performed using a Sysmex XT-2000iV haematology analyser) were performed on samples at room temperature (approximately 22oC) and following warming of the sample to 37oC in a water bath. The Wilcoxon signed rank test was used to compare haematological parameters, including platelet count, before and after sample warming to 37oC. Data are presented as median [25th, 75th percentile] increase. Results: Blood samples from 39 dogs, 19 cats and 10 horses were included. Sample warming to 37oC resulted in a statistically significant increase in platelet counts in dogs (11 [-2, 30] x109/L), cats (36 [14, 84] x109/L) and horses (42 [31, 79] x109/L). Sample warming did not significantly affect other haematological parameters. Conclusions: Pre-warming EDTA blood samples to 37oC prior to haematological analysis reduces the severity of pseudothrombocytopenia in dogs, cats and horses and might negate the need for repeating the platelet count in animals for which determination of an accurate platelet count is essential.
External DOI: https://doi.org/10.1111/vcp.12378
This record's URL: https://www.repository.cam.ac.uk/handle/1810/251463