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dc.contributor.authorCornuault, Valérieen
dc.contributor.authorBuffetto, Fannyen
dc.contributor.authorRydahl, Maja Gen
dc.contributor.authorMarcus, Susan Een
dc.contributor.authorTorode, Thomas Aen
dc.contributor.authorXue, Jieen
dc.contributor.authorCrépeau, Marie-Jeanneen
dc.contributor.authorFaria-Blanc, Nunoen
dc.contributor.authorWillats, William GTen
dc.contributor.authorDupree, Paulen
dc.contributor.authorRalet, Marie-Christineen
dc.contributor.authorKnox, J Paulen
dc.date.accessioned2015-12-09T15:26:03Z
dc.date.available2015-12-09T15:26:03Z
dc.date.issued2015-07-25en
dc.identifier.citationCornuault et al. Planta (2015) Vol. 242, Issue 6, pp. 1321-1334. doi: 10.1007/s00425-015-2375-4en
dc.identifier.issn0032-0935
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/252919
dc.description.abstractMain conclusion The derivation of two sensitive monoclonal antibodies directed to heteroxylan cell wall polysaccharide preparations has allowed the identification of potential inter-linkages between xylan and pectin in potato tuber cell walls and also between xylan and arabinogalactan-proteins in oat grain cell walls. Plant cell walls are complex composites of structurally distinct glycans that are poorly understood in terms of both in muro inter-linkages and developmental functions. Monoclonal antibodies (MAbs) are versatile tools that can detect cell wall glycans with high sensitivity through the specific recognition of oligosaccharide structures. The isolation of two novel MAbs, LM27 and LM28, directed to heteroxylan, subsequent to immunisation with a potato cell wall fraction enriched in rhamnogalacturonan-I (RG-I) oligosaccharides, is described. LM27 binds strongly to heteroxylan preparations from grass cell walls and LM28 binds to a glucuronosyl-containing epitope widely present in heteroxylans. Evidence is presented suggesting that in potato tuber cell walls, some glucuronoxylan may be linked to pectic macromolecules. Evidence is also presented that suggests in oat spelt xylan both the LM27 and LM28 epitopes are linked to arabinogalactan-proteins as tracked by the LM2 arabinogalactan-protein epitope. This work extends knowledge of the potential occurrence of inter-glycan links within plant cell walls and describes molecular tools for the further analysis of such links.
dc.description.sponsorshipThis work was supported by the European Union Seventh Framework Programme (FP7 2007-2013) under the WallTraC project (Grant Agreement number 263916). (This article reflects the authors’ views only and the European Union is not liable for any use that may be made of the information contained herein). The work was also supported by the United Kingdom Biotechnology and Biological Research Council (BBSRC, Grant BB/K017489/1). JX acknowledges support from the Chinese Scholarship Council, TAT from a BBSRC studentship and MGR from the Danish Strategic Research Council and The Danish Council for Independent Research, Technology and Production Sciences as part of the GlycAct project (FI 10-093465). We acknowledge kind gifts of enzymes from Harry Gilbert and oligosaccharides from Sanna Koutaniemi. We thank Theodora Tryfona for mass spectrometry analysis.
dc.languageEnglishen
dc.language.isoenen
dc.publisherSpringer
dc.rightsAttribution 2.0 UK: England & Wales*
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/uk/*
dc.subjectArabinogalactan-proteinsen
dc.subjectcell wallen
dc.subjectGlucuronoxylanen
dc.subjectpectinen
dc.subjectpolysaccharidesen
dc.subjectRhamnogalacturonan-Ien
dc.titleMonoclonal antibodies indicate low-abundance links between heteroxylan and other glycans of plant cell wallsen
dc.typeArticle
dc.description.versionThis is the final version of the article. It first appeared from Springer via http://dx.doi.org/10.1007/s00425-015-2375-4en
prism.endingPage1334
prism.publicationDate2015en
prism.publicationNamePlantaen
prism.startingPage1321
prism.volume242en
dc.rioxxterms.funderBBSRC
dc.rioxxterms.projectidBB/K017489/1
dcterms.dateAccepted2015-07-15en
rioxxterms.versionofrecord10.1007/s00425-015-2375-4en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2015-07-25en
dc.contributor.orcidDupree, Paul [0000-0001-9270-6286]
dc.identifier.eissn1432-2048
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idBBSRC (BB/G016240/1)


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Attribution 2.0 UK: England & Wales
Except where otherwise noted, this item's licence is described as Attribution 2.0 UK: England & Wales