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dc.contributor.authorUntiveros, Miltonen
dc.contributor.authorOlspert, Allanen
dc.contributor.authorArtola, Katrinen
dc.contributor.authorFirth, Andrewen
dc.contributor.authorKreuze, Jan Fen
dc.contributor.authorValkonen, Jari PTen
dc.date.accessioned2015-12-22T11:05:25Z
dc.date.available2015-12-22T11:05:25Z
dc.date.issued2016-04-28en
dc.identifier.citationUntiveros et al. Molecular Plant Pathology (2016). doi: 10.1111/mpp.12366en
dc.identifier.issn1464-6722
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/253087
dc.description.abstractThe single-stranded, positive-sense RNA genome of viruses in the genus Potyvirus encodes a large polyprotein that is cleaved to yield 10 mature proteins. The first three cleavage products are P1, HCpro and P3. An additional short open reading frame (ORF), called pipo, overlaps the P3 region of the polyprotein ORF. Four related potyviruses infecting sweet potato (Ipomoea batatas) are predicted to contain a third ORF, called pispo, which overlaps the 3′ third of the P1 region. Recently, pipo has been shown to be expressed via polymerase slippage at a conserved GA6 sequence. Here, we show that pispo is also expressed via polymerase slippage at a GA6 sequence, with higher slippage efficiency (∼5%) than at the pipo site (∼1%). Transient expression of recombinant P1 or the ‘transframe’ product, P1N-PISPO, in Nicotiana benthamiana suppressed local RNA silencing (RNAi), but only P1N-PISPO inhibited short-distance movement of the silencing signal. These results reveal that polymerase slippage in potyviruses is not limited to pipo expression, but can be co-opted for the evolution and expression of further novel gene products.
dc.description.sponsorshipSegundo Fuentes is acknowledged for technical assistance. Work in the AEF lab was funded by grants from the Wellcome Trust (088789 and 106207) and the Biotechnology and Biological Sciences Research Council (BBSRC) (BB/J007072/1 and BB/J015652/1). Work at the University of Helsinki was funded by the Academy of Finland (1134335 and 1276136) and work at CIP was funded by the CGIAR consortium research program for roots, tubers and bananas.
dc.languageEnglishen
dc.language.isoenen
dc.publisherWiley
dc.rightsAttribution 2.0 UK: England & Wales
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/uk/
dc.subjectRNA virusen
dc.subjectgene expressionen
dc.subjecttranscriptional slippageen
dc.subjectframeshiften
dc.subjectoverlapping geneen
dc.subjectRNA silencing suppressionen
dc.titleA novel sweet potato potyvirus open reading frame (ORF) is expressed via polymerase slippage and suppresses RNA silencingen
dc.typeArticle
dc.description.versionThis is the final version of the article. It first appeared from Wiley via http://dx.doi.org/10.1111/mpp.12366en
prism.publicationDate2016en
prism.publicationNameMolecular Plant Pathologyen
dc.rioxxterms.funderWellcome Trust
dc.rioxxterms.funderBBSRC
dc.rioxxterms.projectid088789
dc.rioxxterms.projectid106207
dc.rioxxterms.projectidBB/J007072/1
dc.rioxxterms.projectidBB/J015652/1
dcterms.dateAccepted2015-12-17en
rioxxterms.versionofrecord10.1111/mpp.12366en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2016-04-28en
dc.contributor.orcidFirth, Andrew [0000-0002-7986-9520]
dc.identifier.eissn1364-3703
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWELLCOME TRUST (106207/Z/14/Z)
pubs.funder-project-idBBSRC (BB/J015652/1)
pubs.funder-project-idBBSRC (BB/J007072/1)
pubs.funder-project-idWellcome Trust (088789/Z/09/Z)
cam.orpheus.successThu Jan 30 12:55:35 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2300-01-01


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Attribution 2.0 UK: England & Wales
Except where otherwise noted, this item's licence is described as Attribution 2.0 UK: England & Wales