Analysis of the expression patterns, Subcellular localisations and interaction partners of drosophila proteins using a pigp protein trap library
View / Open Files
Authors
Lowe, Nick
Roote, John
Ryder, Ed
Armean, Irina M
Johnson, Glynnis
Drummond, Emma
Spriggs, Helen
Drummond, Jenny
Magbanua, Jose P
Naylor, Huw
Bastock, Rebecca
Huelsmann, Sven
Trovisco Gradissimo, Vitor
Knowles-Barley, Seymour
Armstrong, J Douglas
White-Cooper, Helen
Hansen, Celia
Phillips, Roger G
The, UK Drosophila Protein Trap Screening Consortium
Publication Date
2014-10-07Journal Title
Development
ISSN
0950-1991
Publisher
The Company of Biologists
Volume
141
Pages
3994-4005
Language
English
Type
Article
Metadata
Show full item recordCitation
Lowe, N., Rees, J., Roote, J., Ryder, E., Armean, I. M., Johnson, G., Drummond, E., et al. (2014). Analysis of the expression patterns, Subcellular localisations and interaction partners of drosophila proteins using a pigp protein trap library. Development, 141 3994-4005. https://doi.org/10.1242/dev.111054
Abstract
Although we now have a wealth of information on the transcription patterns of all the genes in the Drosophila genome, much less is known about the properties of the encoded proteins. To provide information on the expression patterns and subcellular localisations of many proteins in parallel, we have performed a large-scale protein trap screen using a hybrid piggyBac vector carrying an artificial exon encoding yellow fluorescent protein (YFP) and protein affinity tags. From screening 41 million embryos, we recovered 616 verified independent YFP-positive lines representing protein traps in 374 genes, two-thirds of which had not been tagged in previousPelement protein trap screens. Over 20 different research groupsthen characterized the expression patterns of the tagged proteins in a variety of tissues and at several developmental stages. In parallel, we purified many of the tagged proteins from embryos using the affinity tags and identified co-purifying proteins by mass spectrometry. The fly stocks are publicly available through the Kyoto Drosophila Genetics Resource Center. All our data are available via an open access database (Flannotator), which provides comprehensive information on the expression patterns, subcellular localisations and in vivo interaction partners of the trapped proteins. Our resource substantially increases the number of available protein traps in Drosophila and identifies new markers for cellular organelles and structures.
Keywords
Affinity purification, Cytoophidia, Live imaging, piggyBac, Protein trap
Sponsorship
This work was supported by a project grant from the Wellcome Trust [076739], by a Wellcome Trust Principal Research Fellowship to D.StJ. [049818 and 080007], and by core support from the Wellcome Trust [092096] and Cancer Research UK [A14492].
Funder references
Cancer Research UK (A14492)
BBSRC (BB/F010303/1)
Wellcome Trust (086451/Z/08/Z)
Wellcome Trust (092545/Z/10/Z)
Wellcome Trust (099234/Z/12/Z)
WELLCOME TRUST (103792/Z/14/Z)
Wellcome Trust (092096/Z/10/Z)
Wellcome Trust (080007/Z/06/Z)
Wellcome Trust (076739/Z/05/Z)
Wellcome Trust (049818/Z/96/B)
Identifiers
External DOI: https://doi.org/10.1242/dev.111054
This record's URL: https://www.repository.cam.ac.uk/handle/1810/253317
Rights
Attribution 2.0 UK: England & Wales
Licence URL: http://creativecommons.org/licenses/by/2.0/uk/
Recommended or similar items
The following licence files are associated with this item: