Preparation, characterization and in vitro response of bioactive coatings on polyether ether ketone
Publication Date
2015-11-27Journal Title
Journal of Biomedical Materials Research Part B: Applied Biomaterials
ISSN
1552-4973
Publisher
Wiley
Language
English
Type
Article
Metadata
Show full item recordCitation
Durham, J. W. I., Allen, M., & Rabiei, A. (2015). Preparation, characterization and in vitro response of bioactive coatings on polyether ether ketone. Journal of Biomedical Materials Research Part B: Applied Biomaterials https://doi.org/10.1002/jbm.b.33578
Abstract
Polyether ether ketone (PEEK) is a highly heat-resistant thermoplastic with excellent strength and elastic modulus similar to human bone, making it an attractive material for orthopedic implants. However, the hydrophobic surface of PEEK implants induces fibrous encapsulation which is unfavorable for stable implant anchorage. In this study, PEEK was coated via ion-beam-assisted deposition (IBAD) using a two-layer design of yttria-stabilized zirconia (YSZ) as a heat-protection layer, and hydroxyapatite (HA) as a top layer to improve osseointegration. Microstructural analysis of the coatings showed a dense, uniform columnar grain structure in the YSZ layer and no delamination from the substrate. The HA layer was found to be amorphous and free of porosities in its as-deposited state. Subsequent heat treatment via microwave energy followed by autoclaving crystallized the HA layer, confirmed by SEM and XRD analysis. An in vitro study using MC3T3 preosteoblast cells showed improved bioactivity in heat-treated sample groups. Cell proliferation, differentiation, and mineralization were analyzed by MTT assay and DNA content, osteocalcin expression, and Alizarin Red S (AR-S) content, respectively. Initial cell growth was increased, and osteogenic maturation and mineralization were accelerated most on coatings that underwent a combined microwave and autoclave heat treatment process as compared to uncoated PEEK and amorphous HA surfaces.
Keywords
Bioactive coatings, Hydroxyapatite, PEEK, Physical vapor deposition, Crystallinity
Sponsorship
Funded by National Institute of Dental and Craniofacial Research of the National Institutes of Health. Grant Number: R21DE022925
Identifiers
External DOI: https://doi.org/10.1002/jbm.b.33578
This record's URL: https://www.repository.cam.ac.uk/handle/1810/253494
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