Show simple item record

dc.contributor.authorParolini, Lucia
dc.contributor.authorKotar, Jurij
dc.contributor.authorDi Michele, Lorenzo
dc.contributor.authorMognetti, Bortolo M.
dc.date.accessioned2016-02-03T12:56:47Z
dc.date.available2016-02-03T12:56:47Z
dc.date.issued2016
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/253615
dc.descriptionThe dataset contains data on aggregation and melting experiments in samples of DNA-functionalised liposomes as described in the paper "Controlling Self-Assembly Kinetics of DNA-Functionalized Liposomes Using Toehold Exchange Mechanism.” (ACS Nano doi:10.1021/acsnano.5b07201). Each compressed folder labelled as "AGG_TQxxC_Ryyy.zip" contains raw data on aggregation experiments of samples with stoichiometric ratio R=yyy (yyy=0.00, 0.25, 0.50, 0.75, 1.00) incubated at TQ=xx (xx=35, 30, 25, 20, 15) degrees Celsius. Please refer to the paper for details. The data are full resolution epifluorescence microscopy images in .tiff format. Individual images are labelled as "Black_N", "ImZ1_N", "ImZ2_N", and "ImZ3_N". The integer index N=1,...,NMax indicates time, with NMax being different from sample to sample. The physical time corresponding to each time-step is reported in the text file “time.dat" as a string of comma separated numbers. Each compressed “AGG_TQxxC_Ryyy.zip" folder contains the corresponding “time.dat" file. Images labelled as "Black_N" are captured with the fluorescence illumination disabled, ad used for subtracting background signal. Images labelled as "ImZ1_N", "ImZ2_N", and "ImZ3_N" contain snapshots of the samples taken at 3 different vertical positions spaced by 1.00 microns from each other. The data on the manuscript are obtained using the average between these three images diminished by the corresponding "Black_N”. Compressed folders labelled "MELT_TQxxC_Ryyy.zip" contain data on the melting experiments on the same samples of the corresponding aggregation experiments. Image files inside are named according to the same scheme, but here the index “N" corresponds different temperatures, increasing stepwise from TQ. The sample temperature measured for each "N" is reported in the corresponding text file “TemperatureRamp_TQxxC.dat" in comma-separated format. Note that the temperature files TemperatureRamp_TQxxC.dat are not included in the compressed folders “MELT_TQxxC_Ryyy.zip” and need to be downloaded separately. The data shown in Figure 2 of the paper are obtained by averaging over three similar datasets. Analysis methods are described in the Methods section of the paper.en
dc.descriptionThis record is pending publication in ACS Nano ("Controlling Self-Assembly Kinetics of DNA-Functionalized Liposomes Using Toehold Exchange Mechanism. Experimental dataset."). This record is embargoed until publication and it will be updated.
dc.description.sponsorshipThis work was supported by the EPSRC [grant number EP/J017566/1].en
dc.formatThe image files are in .tiff formaten
dc.publisherUniversity of Cambridgeen
dc.rightsAttribution 2.0 UK: England & Wales*
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/uk/*
dc.subjectDNAen
dc.subjectkineticsen
dc.subjectself-assemblyen
dc.subjectliposomesen
dc.subjecttoe-holdingen
dc.subjectaggregationen
dc.titleResearch data supporting "Controlling Self-Assembly Kinetics of DNA-Functionalized Liposomes Using Toehold Exchange Mechanism"en
dc.typeDataseten
dc.rioxxterms.funderEPSRC
dc.rioxxterms.projectidEP/J017566/1
pubs.declined2017-10-11T13:54:36.892+0100
dcterms.formattiff
dc.contributor.orcidDi Michele, Lorenzo [0000-0002-1458-9747]
datacite.issupplementto.doi10.1021/acsnano.5b07201


Files in this item

Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution 2.0 UK: England & Wales
Except where otherwise noted, this item's licence is described as Attribution 2.0 UK: England & Wales