The stable isotope compositions of moss tissue water (δ$\mathrm{<mrow\; data-mjx-texclass="ORD">2</mrow>}$H and δ$\mathrm{<mrow\; data-mjx-texclass="ORD">18</mrow>}$O) and cellulose (δ$\mathrm{<mrow\; data-mjx-texclass="ORD">13</mrow>}$C and δ$\mathrm{<mrow\; data-mjx-texclass="ORD">18</mrow>}$O), and testate amoebae populations were sampled from 61 contemporary surface samples along a 600-km latitudinal gradient of the Antarctic Peninsula (AP) to provide a spatial record of environmental change. The isotopic composition of moss tissue water represented an annually integrated precipitation signal with the expected isotopic depletion with increasing latitude. There was a weak, but significant, relationship between cellulose δ$\mathrm{<mrow\; data-mjx-texclass="ORD">18</mrow>}$O and latitude, with predicted source water inputs isotopically enriched compared to measured precipitation. Cellulose δ$\mathrm{<mrow\; data-mjx-texclass="ORD">13</mrow>}$C values were dependent on moss species and water content, and may reflect site exposure to strong winds. Testate amoebae assemblages were characterised by low concentrations and taxonomic diversity, with $Corythion\; dubium$ and $Microcorycia\; radiata$ types the most cosmopolitan taxa. The similarity between the intra- and inter-site ranges measured in all proxies suggests that microclimate and micro-topographical conditions around the moss surface were important determinants of proxy values. Isotope and testate amoebae analyses have proven value as palaeoclimatic, temporal proxies of climate change, whereas this study demonstrates that variations in isotopic and amoeboid proxies between microsites can be beyond the bounds of the current spatial variability in AP climate.