Quantitative neuroanatomy for connectomics in Drosophila
Schneider-Mizell, Casey M
Zwart, Maarten F
Midgley, Frank M
Fetter, Richard D
MetadataShow full item record
Schneider-Mizell, C. M., Gerhard, S., Longair, M., Kazimiers, T., Li, F., Zwart, M. F., Champion, A., et al. (2016). Quantitative neuroanatomy for connectomics in Drosophila. eLife, 5 (e12059)https://doi.org/10.7554/eLife.12059
Neuronal circuit mapping using electron microscopy demands laborious proofreading or reconciliation of multiple independent reconstructions. Here, we describe new methods to apply quantitative arbor and network context to iteratively proofread and reconstruct circuits and create anatomically enriched wiring diagrams. We measured the morphological underpinnings of connectivity in new and existing reconstructions of Drosophila sensorimotor (larva) and visual (adult) systems. Synaptic inputs were preferentially located on numerous small, microtubule-free 'twigs' which branch off a single microtubule-containing 'backbone'. Omission of individual twigs accounted for 96% of errors. However, the synapses of highly connected neurons were distributed across multiple twigs. Thus, the robustness of a strong connection to detailed twig anatomy was associated with robustness to reconstruction error. By comparing iterative reconstruction to the consensus of multiple reconstructions, we show that our method overcomes the need for redundant effort through the discovery and application of relationships between cellular neuroanatomy and synaptic connectivity.
Funding came from the HHMI Janelia Visiting Scientist program (AC), Swiss National Science Foundation grant 31003A 132969 (AC), HHMI, and the Institute of Neuroinformatics of the University of Zurich and ETH Zurich.
External DOI: https://doi.org/10.7554/eLife.12059
This record's URL: https://www.repository.cam.ac.uk/handle/1810/254783
Attribution 2.0 UK: England & Wales
Licence URL: http://creativecommons.org/licenses/by/2.0/uk/
Recommended or similar items
The following licence files are associated with this item: