Show simple item record

dc.contributor.authorKulish, Oen
dc.contributor.authorWright, ADen
dc.contributor.authorTerentjev, Eugeneen
dc.date.accessioned2016-09-12T15:12:36Z
dc.date.available2016-09-12T15:12:36Z
dc.date.issued2016-06-20en
dc.identifier.issn2045-2322
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/260141
dc.description.abstractF1F0 ATP synthase (ATPase) either facilitates the synthesis of ATP in a process driven by the proton moving force (pmf), or uses the energy from ATP hydrolysis to pump protons against the concentration gradient across the membrane. ATPase is composed of two rotary motors, F0 and F1, which compete for control of their shared γ -shaft. We present a self-consistent physical model of F1 motor as a simplified two-state Brownian ratchet using the asymmetry of torsional elastic energy of the coiled-coil γ -shaft. This stochastic model unifies the physical concepts of linear and rotary motors, and explains the stepped unidirectional rotary motion. Substituting the model parameters, all independently known from recent experiments, our model quantitatively reproduces the ATPase operation, e.g. the 'no-load' angular velocity is ca. 400 rad/s anticlockwise at 4 mM ATP. Increasing the pmf torque exerted by F0 can slow, stop and overcome the torque generated by F1, switching from ATP hydrolysis to synthesis at a very low value of 'stall torque'. We discuss the motor efficiency, which is very low if calculated from the useful mechanical work it produces - but is quite high when the 'useful outcome' is measured in the number of H(+) pushed against the chemical gradient.
dc.languageengen
dc.language.isoenen
dc.rightsAttribution 4.0 International*
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleF1 rotary motor of ATP synthase is driven by the torsionally-asymmetric drive shaft.en
dc.typeArticle
prism.endingPage28180
prism.publicationDate2016en
prism.publicationNameSci Repen
prism.startingPage28180
prism.volume6en
dc.identifier.doi10.17863/CAM.4367
dcterms.dateAccepted2016-05-31en
rioxxterms.versionofrecord10.1038/srep28180en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2016-06-20en
dc.contributor.orcidTerentjev, Eugene [0000-0003-3517-6578]
dc.identifier.eissn2045-2322
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idEPSRC (EP/F032773/1)
pubs.funder-project-idEPSRC (EP/J017639/1)


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International