Synthesis and biophysical analysis of modified thymine-containing DNA oligonucleotides.
Accepted version
Peer-reviewed
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Repository DOI
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Authors
Kawasaki, F
Murat, P
Li, Z
Santner, T
Abstract
We report the synthesis of a 5-formyl-2'-deoxyuridine (5fU) phosphoramidite and the preparation of oligonucleotides comprising all known, naturally observed eukaryotic thymidine modifications. Biophysical characterization of the synthetic oligonucleotides indicates that 5fU, but not the other T-derivatives, can alter DNA structures.
Description
Keywords
DNA, Deoxyuridine, Oligonucleotides, Organophosphorus Compounds, Thymine
Journal Title
Chemical Communications
Conference Name
Journal ISSN
1359-7345
1364-548X
1364-548X
Volume Title
53
Publisher
Royal Society of Chemistry
Publisher DOI
Sponsorship
Wellcome Trust (099232/Z/12/Z)
This work was supported by the following grants: the Wellcome Trust (099232/Z/12/Z), Cancer Research UK (C14303/A17197), ERC Advanced grant (PM), Herchel Smith Funding and A*STAR (ZL).