Non-coding Y RNAs associate with early replicating euchromatin in concordance with the origin recognition complex
Journal of Cell Science
The Company of Biologists Ltd.
MetadataShow full item record
Kheir, E., & Krude, T. (2017). Non-coding Y RNAs associate with early replicating euchromatin in concordance with the origin recognition complex. Journal of Cell Science, 130 (7), 1239-1250. https://doi.org/10.1242/jcs.197566
Non-coding Y RNAs are essential for the initiation of chromosomal DNA replication in vertebrates, yet their association with chromatin during the cell cycle is not characterised. Here, we quantify human Y RNA levels in soluble and chromatin-associated intracellular fractions and investigate, topographically, their dynamic association with chromatin during the cell cycle. We find that, on average, about a million Y RNA molecules are present in the soluble fraction of a proliferating cell, and 5-10-fold less are in association with chromatin. These levels decrease substantially during quiescence. No significant differences are apparent between cancer and non-cancer cell lines. Y RNAs associate with euchromatin throughout the cell cycle. Their levels are 2-4-fold higher in S phase than in G1 phase or mitosis. Y RNAs are not detectable at active DNA replication foci, and re-associate with replicated euchromatin during mid and late S phase. The dynamics and sites of Y1 RNA association with chromatin are in concordance with those of the origin recognition complex (ORC). Our data therefore suggest a functional role of Y RNAs in a common pathway with ORC.
non-coding Y RNA, origin recognition complex, DNA replication, ORC, initiation, chromatin association, cell cycle
This work was supported by the Association for International Cancer Research (AICR) (project grant 10-0570).
Worldwide Cancer Research (10-0570)
External DOI: https://doi.org/10.1242/jcs.197566
This record's URL: https://www.repository.cam.ac.uk/handle/1810/263953