Albugo-imposed changes to tryptophan-derived antimicrobial metabolite biosynthesis may contribute to suppression of non-host resistance to Phytophthora infestans in Arabidopsis thaliana
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Authors
Prince, David C
Rallapalli, Ghanasyam
Xu, Deyang
Schoonbeek, Henk-jan
Çevik, Volkan
Asai, Shuta
Kemen, Eric
Cruz-Mireles, Neftaly
Kemen, Ariane
Belhaj, Khaoula
Kamoun, Sophien
Holub, Eric B
Halkier, Barbara A
Jones, Jonathan DG
Publication Date
2017-03-20ISSN
1741-7007
Type
Article
Metadata
Show full item recordCitation
Prince, D. C., Rallapalli, G., Xu, D., Schoonbeek, H., Çevik, V., Asai, S., Kemen, E., et al. (2017). Albugo-imposed changes to tryptophan-derived antimicrobial metabolite biosynthesis may contribute to suppression of non-host resistance to Phytophthora infestans in Arabidopsis thaliana. https://doi.org/10.1186/s12915-017-0360-z
Abstract
Abstract
Background
Plants are exposed to diverse pathogens and pests, yet most plants are resistant to most plant pathogens. Non-host resistance describes the ability of all members of a plant species to successfully prevent colonization by any given member of a pathogen species. White blister rust caused by Albugo species can overcome non-host resistance and enable secondary infection and reproduction of usually non-virulent pathogens, including the potato late blight pathogen Phytophthora infestans on Arabidopsis thaliana. However, the molecular basis of host defense suppression in this complex plant–microbe interaction is unclear. Here, we investigate specific defense mechanisms in Arabidopsis that are suppressed by Albugo infection.
Results
Gene expression profiling revealed that two species of Albugo upregulate genes associated with tryptophan-derived antimicrobial metabolites in Arabidopsis. Albugo laibachii-infected tissue has altered levels of these metabolites, with lower indol-3-yl methylglucosinolate and higher camalexin accumulation than uninfected tissue. We investigated the contribution of these Albugo-imposed phenotypes to suppression of non-host resistance to P. infestans. Absence of tryptophan-derived antimicrobial compounds enables P. infestans colonization of Arabidopsis, although to a lesser extent than Albugo-infected tissue. A. laibachii also suppresses a subset of genes regulated by salicylic acid; however, salicylic acid plays only a minor role in non-host resistance to P. infestans.
Conclusions
Albugo sp. alter tryptophan-derived metabolites and suppress elements of the responses to salicylic acid in Arabidopsis. Albugo sp. imposed alterations in tryptophan-derived metabolites may play a role in Arabidopsis non-host resistance to P. infestans. Understanding the basis of non-host resistance to pathogens such as P. infestans could assist in development of strategies to elevate food security.
Sponsorship
Gatsby Charitable Foundation (unknown)
Identifiers
External DOI: https://doi.org/10.1186/s12915-017-0360-z
This record's URL: https://www.repository.cam.ac.uk/handle/1810/264064
Rights
Rights Holder: Jones et al.
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